Aslan Enes, Vyas Cian, Yupanqui Mieles Joel, Humphreys Gavin, Diver Carl, Bartolo Paulo
Department of Machine and Metal Technologies, Gumusova Vocational School, Duzce University, Duzce 81850, Turkey.
Department of Mechanical, Aerospace and Civil Engineering, University of Manchester, Oxford Road, Manchester M13 9PL, UK.
Materials (Basel). 2021 Dec 23;15(1):89. doi: 10.3390/ma15010089.
Skin is a hierarchical and multi-cellular organ exposed to the external environment with a key protective and regulatory role. Wounds caused by disease and trauma can lead to a loss of function, which can be debilitating and even cause death. Accelerating the natural skin healing process and minimizing the risk of infection is a clinical challenge. Electrospinning is a key technology in the development of wound dressings and skin substitutes as it enables extracellular matrix-mimicking fibrous structures and delivery of bioactive materials. Honey is a promising biomaterial for use in skin tissue engineering applications and has antimicrobial properties and potential tissue regenerative properties. This preliminary study investigates a solution electrospun composite nanofibrous mesh based on polycaprolactone and a medical grade honey, SurgihoneyRO. The processing conditions were optimized and assessed by scanning electron microscopy to fabricate meshes with uniform fiber diameters and minimal presence of beads. The chemistry of the composite meshes was examined using Fourier transform infrared spectroscopy and X-ray photon spectroscopy showing incorporation of honey into the polymer matrix. Meshes incorporating honey had lower mechanical properties due to lower polymer content but were more hydrophilic, resulting in an increase in swelling and an accelerated degradation profile. The biocompatibility of the meshes was assessed using human dermal fibroblasts and adipose-derived stem cells, which showed comparable or higher cell metabolic activity and viability for SurgihoneyRO-containing meshes compared to polycaprolactone only meshes. The meshes showed no antibacterial properties in a disk diffusion test due to a lack of hydrogen peroxide production and release. The developed polycaprolactone-honey nanofibrous meshes have potential for use in skin applications.
皮肤是一个分层的多细胞器官，暴露于外部环境中，具有关键的保护和调节作用。疾病和创伤导致的伤口会导致功能丧失，这可能使人衰弱甚至导致死亡。加速皮肤自然愈合过程并将感染风险降至最低是一项临床挑战。电纺丝是伤口敷料和皮肤替代物开发中的一项关键技术，因为它能够制造模仿细胞外基质的纤维结构并递送生物活性材料。蜂蜜是一种有前途的生物材料，可用于皮肤组织工程应用，具有抗菌特性和潜在的组织再生特性。这项初步研究调查了一种基于聚己内酯和医用级蜂蜜SurgihoneyRO的溶液电纺复合纳米纤维网。通过扫描电子显微镜对加工条件进行了优化和评估，以制造具有均匀纤维直径且珠子最少的网。使用傅里叶变换红外光谱和X射线光子光谱对复合网的化学性质进行了检查，结果表明蜂蜜已掺入聚合物基质中。由于聚合物含量较低，掺入蜂蜜的网的机械性能较低，但亲水性更强，导致膨胀增加和降解曲线加快。使用人真皮成纤维细胞和脂肪来源的干细胞评估了网的生物相容性，结果表明，与仅含聚己内酯的网相比，含SurgihoneyRO的网具有相当或更高的细胞代谢活性和活力。由于缺乏过氧化氢的产生和释放，这些网在纸片扩散试验中没有显示出抗菌性能。所开发的聚己内酯-蜂蜜纳米纤维网具有用于皮肤应用的潜力。
