Wang Xiaomeng, Li Wenpei, Xu Mengjia, Tian Juanjuan, Li Wei
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Foods. 2021 Dec 21;11(1):12. doi: 10.3390/foods11010012.
In this study, a high-throughput sequencing technique was used to analyze bacterial and fungal diversity of two traditional Tibetan kefir grains from Linzhi (K1) and Naqu (K2) regions. Comparative bioinformatic analyses indicated that , and were the main dominant strains in K1 and K2. In order to research the relationship of the growth of kefir grains, the biofilm and the extracellular polysaccharides (EPS) produced by microorganisms, the proliferation rate of kefir grains, the yield and chemical structure of EPS and the optimal days for biofilm formation were determined. The results showed that the growth rate, the yield of EPS and the biofilm formation ability of K1 were higher than K2, and the optimal day of their biofilm formation was the same in 10th day. Additionally, the live cells, dead cells and EPS in biofilm formation of K1 and K2 were observed by fluorescence microscope to clarify the formation process of kefir grains. To determine the influence of microbial interactions on biofilm and the formation of kefir grains, the essential role of microbial quorum sensing needs further attention.
在本研究中，采用高通量测序技术分析了来自林芝（K1）和那曲（K2）地区的两种传统藏式开菲尔粒的细菌和真菌多样性。比较生物信息学分析表明， 、 和 是K1和K2中的主要优势菌株。为了研究开菲尔粒的生长、微生物产生的生物膜和胞外多糖（EPS）之间的关系，测定了开菲尔粒的增殖率、EPS的产量和化学结构以及生物膜形成的最佳天数。结果表明，K1的生长速率、EPS产量和生物膜形成能力均高于K2，且它们生物膜形成 的最佳天数均为第10天。此外，通过荧光显微镜观察了K1和K2生物膜形成过程中的活细胞、死细胞和EPS，以阐明开菲尔粒的形成过程。为了确定微生物相互作用对生物膜和开菲尔粒形成的影响，微生物群体感应的重要作用需要进一步关注。
