Center of Exact and Technological Sciences - CCET, State University of West Paraná, Cascavel, Brazil.
Department of Bioprocess Engineering and Biotechnology - COEBB/TD, Federal University of Technology - Paraná (UTFPR), Toledo, Brazil.
Crit Rev Biotechnol. 2021 Dec;41(8):1131-1152. doi: 10.1080/07388551.2021.1917505. Epub 2021 May 2.
is an ascomycetous yeast which has shown promising results in cellulosic ethanol and renewable chemicals production. It can survive on a variety of carbon sources under industrially favorable conditions due to its fast growth rate, thermotolerance, and acid tolerance. is generally regarded as a safe (GRAS) microorganism, is widely recognized as a powerhouse for the production of heterologous proteins and is accepted by the US Food and Drug Administration (USFDA) for its pharmaceutical and food applications. Since lignocellulosic hydrolysates are comprised of diverse monomeric sugars, oligosaccharides and potential metabolism inhibiting compounds, this microorganism can play a pivotal role as it can grow on lignocellulosic hydrolysates coping with vegetal cell wall derived inhibitors. Furthermore, advancements in synthetic biology, for example CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats with Cas9)-mediated genome editing, will enable development of an engineered yeast for the production of biochemicals and biopharmaceuticals having a myriad of industrial applications. Genetic engineering companies such as Cargill, Ginkgo Bioworks, DuPont, Global Yeast, Genomatica, and several others are actively working to develop designer yeasts. Given the important traits and properties of , these companies may find it to be a suitable biocatalyst for renewable chemicals and fuel production on the large scale. This paper reviews the recent progress made with biotechnology for sustainable production of ethanol, and other products utilizing lignocellulosic sugars.
是一种子囊菌酵母，在纤维素乙醇和可再生化学品生产方面显示出了有前景的结果。由于其快速的生长速度、耐热性和耐酸性，它可以在工业上有利的条件下利用各种碳源生存。被普遍认为是一种安全（GRAS）的微生物，被广泛认为是生产异源蛋白的有力工具，并且因其在制药和食品方面的应用而被美国食品和药物管理局（USFDA）接受。由于木质纤维素水解物由不同的单体糖、寡糖和潜在的代谢抑制化合物组成，因此该微生物可以发挥关键作用，因为它可以在木质纤维素水解物上生长，同时应对植物细胞壁衍生的抑制剂。此外，合成生物学的进步，例如 CRISPR-Cas9（成簇规律间隔短回文重复序列与 Cas9）介导的基因组编辑，将使开发用于生产具有无数工业应用的生物化学物质和生物制药的工程酵母成为可能。嘉吉、银杏生物工程、杜邦、环球酵母、Genomatica 等基因工程公司正在积极努力开发设计酵母。鉴于的重要特征和性质，这些公司可能会发现它是一种适合大规模生产可再生化学品和燃料的生物催化剂。本文综述了利用木质纤维素糖可持续生产乙醇和其他产品的生物技术的最新进展。
