College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
College of Food Science and Engineering, Nanjing University of Finance and Economic, Nanjing, Jiangsu 210023, PR China.
Int J Biol Macromol. 2021 Jul 31;183:1034-1046. doi: 10.1016/j.ijbiomac.2021.05.047. Epub 2021 May 8.
Two exopolysaccharide fractions (EPS1 and EPS2) were obtained from Paecilomyces cicadae TJJ 1213, and their structures were elucidated. The EPS1 and EPS2 were mainly composed of mannose and galactose with molar ratios of 3.2: 1.0 and 2.7: 1.0, respectively. They possessed average molecular weights of 1.69 × 10 and 8.06 × 10 Da, respectively. Structural characterization indicated that the backbone of EPS1 was consisted of →4)-α-D-Manp (1→, →3,4)-α-D-Manp (1 → and →2,6)-α-D-Manp (1→, →6)-α-D-Galp (1→, →6)-β-D-Galp (1→, and side chain was consisted of α-D-Manp residue. The backbone of EPS2 was composed of →6)-β-D-Galp-(1→, →4)-α-D-Manp-(1→, →2,6)-α-D-Manp-(1 → and →6)-α-D-Galp-(1→, and the branching point was also consisted of α-D-Manp residue. In addition, EPS1 and EPS2 had potential in protective effects of PC12 cells against hydrogen peroxide induced oxidative stress by inhibiting the production of ROS, reducing LDH leakage and alleviating mitochondrial damage. These results indicated that EPS1 and EPS2 might serve as therapeutic agents for neuronal disorders.
从蝉拟青霉 TJJ1213 中得到了两种胞外多糖（EPS1 和 EPS2），并对其结构进行了阐明。EPS1 和 EPS2 主要由甘露糖和半乳糖组成，摩尔比分别为 3.2:1.0 和 2.7:1.0。它们的平均分子量分别为 1.69×10 和 8.06×10 Da。结构表征表明，EPS1 的主链由→4)-α-D-Manp（1→、→3,4）-α-D-Manp（1→和→2,6）-α-D-Manp（1→、→6）-α-D-Galp（1→、→6）-β-D-Galp（1→和侧链组成，侧链由α-D-Manp 残基组成。EPS2 的主链由→6）-β-D-Galp-（1→、→4）-α-D-Manp-（1→、→2,6）-α-D-Manp-（1→和→6）-α-D-Galp-（1→组成，分支点也由α-D-Manp 残基组成。此外，EPS1 和 EPS2 具有通过抑制 ROS 产生、减少 LDH 漏出和缓解线粒体损伤来保护 PC12 细胞免受过氧化氢诱导的氧化应激的潜在作用。这些结果表明，EPS1 和 EPS2 可能作为治疗神经元紊乱的药物。
