Fiorentini Cecilia, Duserm Garrido Guillermo, Bassani Andrea, Cortimiglia Claudia, Zaccone Marta, Montalbano Luana, Martinez-Nogues Vanesa, Cocconcelli Pier Sandro, Spigno Giorgia
Department for Sustainable Food Process (DiSTAS), Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy.
Proplast, Via Roberto di Ferro 86, 15122 Alessandria, Italy.
Foods. 2021 Dec 23;11(1):30. doi: 10.3390/foods11010030.
The thermal stability of four different commercial citrus peel extracts was tested and improved by an encapsulation process with β-cyclodextrins in a spray-dryer. All extracts after the encapsulation process maintained a good antioxidant capacity, with an apparent loss in total phenolic compounds of around 20-25%. In addition, all samples showed good antimicrobial activity (MIC 5-0.625 mg/mL) against , which was maintained after the encapsulation process (MIC 5-1.25 mg/mL). Based on the antioxidant and antimicrobial activity results, the best-encapsulated citrus extract was selected for incorporation into a polylactic acid/polyhydroxy butyrate (PLA/PHB) film. The latter was then produced on an industrial scale by cast extrusion and was found to be suitable for food contact as it showed overall migration values in different food simulants lower than the legislative limit of 10 mg of non-volatile substances per 1 dm of surface area. The UHPLC-HRMS analysis, performed to evaluate the migration of the active compounds, revealed about 13.41% release in food simulant A and 11.02% in food simulant B. Antimicrobial analysis conducted directly on the film showed a growth inhibition activity towards and equal to 30 and 60%, respectively.
对四种不同的市售柑橘皮提取物的热稳定性进行了测试，并通过在喷雾干燥器中用β-环糊精进行包封处理来加以改善。包封处理后的所有提取物均保持了良好的抗氧化能力，总酚类化合物明显损失约20-25%。此外，所有样品对……均表现出良好的抗菌活性（最低抑菌浓度为5-0.625毫克/毫升），包封处理后该活性得以保持（最低抑菌浓度为5-1.25毫克/毫升）。基于抗氧化和抗菌活性结果，选择了包封效果最佳的柑橘提取物掺入聚乳酸/聚羟基丁酸酯（PLA/PHB）薄膜中。然后通过流延挤出在工业规模上生产该薄膜，发现其适用于食品接触，因为它在不同食品模拟物中的总迁移值低于每1平方分米表面积10毫克非挥发性物质的法定限值。为评估活性化合物的迁移而进行的超高效液相色谱-高分辨质谱分析表明，在食品模拟物A中的释放率约为13.41%，在食品模拟物B中的释放率为11.02%。直接在薄膜上进行的抗菌分析表明，对……的生长抑制活性分别为30%和60%。
