κ-卡拉胶在无蛋黄稀释液中的应用提高了犬精液冷冻保存的效率。

The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation.

作者信息

Kim Eunji, Almubarak Areeg, Talha Nabeel, Yu Il-Jeoung, Jeon Yubyeol

机构信息

Department of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Korea.

Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Sudan University of Science and Technology, P.O. Box 204, Hilat Kuku, Khartoum North 11111, Sudan.

出版信息

Animals (Basel). 2021 Dec 31;12(1):88. doi: 10.3390/ani12010088.

DOI:10.3390/ani12010088

PMID:35011194

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8749662/

Abstract

κ-Carrageenan is a plant polysaccharide derived from red seaweeds reported to possess potential medicinal and antioxidants activities. The present study aimed to identify the cryoprotective effects of κ-carrageenan on the quality of frozen-thawed canine semen. Twenty-eight ejaculates were collected and diluted in a Tris egg-yolk-free extender supplemented with various concentrations of κ-carrageenan (0.0%, 0.1%, 0.2%, 0.3%, and 0.5%). The addition of κ-carrageenan to the extender at a 0.2% concentration induced a significant increase in the total motility (TM) and the rapid progressive motility (RPM) of canine sperm. Among the experimental groups, the highest percentage of sperms with intact acrosomes was found in the 0.5% κ-carrageenan group ( < 0.05). Apoptosis levels were significantly lower in the 0.1% and 0.2% κ-carrageenan treatment. Moreover, sperm in the κ-carrageenan supplemented group showed a significantly higher expression of antiapoptotic (Bcl-2) and lower expression of NADPH oxidase (NOX5), spermine synthase (SMS), and spermine oxidase (SMOX) genes than those in the control group. In conclusion, the addition of κ-carrageenan to the freezing extender improved the overall efficiency of frozen-thawed dog spermatozoa.

摘要

κ-卡拉胶是一种从红海藻中提取的植物多糖，据报道具有潜在的药用和抗氧化活性。本研究旨在确定κ-卡拉胶对冻融犬精液质量的冷冻保护作用。收集了28份射精样本，并在添加了不同浓度κ-卡拉胶（0.0%、0.1%、0.2%、0.3%和0.5%）的无 Tris 蛋黄稀释液中进行稀释。在稀释液中添加0.2%浓度的κ-卡拉胶可使犬精子的总活力（TM）和快速前进活力（RPM）显著增加。在实验组中，0.5%κ-卡拉胶组中顶体完整的精子百分比最高（<0.05）。在0.1%和0.2%κ-卡拉胶处理组中，细胞凋亡水平显著降低。此外，与对照组相比，添加κ-卡拉胶组的精子抗凋亡蛋白（Bcl-2）表达显著更高，而烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NOX5）、精胺合成酶（SMS）和精胺氧化酶（SMOX）基因的表达更低。总之，在冷冻稀释液中添加κ-卡拉胶可提高冻融犬精子的整体效率。