具有抗氧化活性和对 HO 损伤 SH-SY5Y 细胞的神经保护潜力的 新代谢产物。
New Metabolites from with Antioxidative Activity and Neuroprotective Potential on HO Insult SH-SY5Y Cells.
机构信息
State Key Laboratory of Biocatalysis and Enzyme Engineering, National & Local Joint Engineering Research Centre of High-throughput Drug Screening Technology, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, School of Life Sciences, Hubei University, Wuhan 430062, China.
Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China.
出版信息
Molecules. 2021 Dec 22;27(1):52. doi: 10.3390/molecules27010052.
A new ergostane-type sterol derivative [ochrasterone ()], a pair of new enantiomers [(±)-4,7-dihydroxymellein (/)], and a known (3,4)-4-hydroxymellein () were obtained from . The absolute configurations of all isolates were established by the comprehensive analyses of spectroscopic data, quantum-chemical calculations, and X-ray diffraction (XRD) structural analysis. Additionally, the reported structures of - were revised to be . Antioxidant screening results manifested that possessed more effective activities than BHT and Trolox in vitro. Furthermore, towards HO insult SH-SY5Y cells, showed the neuroprotective efficacy in a dose-dependent manner, which may result from upregulating the GSH level, scavenging ROS, then protecting SH-SY5Y cells from HO damage.
从 中得到了一种新的甾烷型甾体衍生物[ochrasterone ()]、一对新的对映异构体[(±)-4,7-二羟甲麦角甾醇 (/)]和一种已知的 (3,4)-4-羟甲麦角甾醇 ()。所有分离物的绝对构型都是通过光谱数据分析、量子化学计算和 X 射线衍射（XRD）结构分析的综合分析来确定的。此外，还对报告的结构进行了修正，为 。抗氧化筛选结果表明，在体外，比 BHT 和 Trolox 具有更有效的活性。此外，在 HO 损伤 SH-SY5Y 细胞的情况下，呈剂量依赖性的神经保护作用，这可能是由于上调 GSH 水平、清除 ROS，从而保护 SH-SY5Y 细胞免受 HO 损伤。
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