Li Wei, Zhou Wei, Cha Ji Yun, Kwon Se Uk, Baek Kwang-Hyun, Shim Sang Hee, Lee Young Mi, Kim Young Ho
School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Phytochemistry. 2015 Jul;115:231-8. doi: 10.1016/j.phytochem.2015.02.021. Epub 2015 Mar 18.
Erinarols G-J and 10 known ergostane-type sterols were isolated from a methanol extract of the dried fruiting bodies of Hericium erinaceum. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as well as through comparison with previously reported data. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of tumor necrosis factor α (TNF-α) and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cells. The results showed that erinarols H and J, as well as 2 of the ergostane-type sterols exhibited inhibitory activity against TNF-α secretion, with inhibition values ranging from 33.7% to 43.3% at 10 μM. Erinarols J and three ergostane-type sterols exhibited significant inhibitory effects against NO production, with inhibition values ranging from 38.4% to 71.5% at 10 μM.
从猴头菇干燥子实体的甲醇提取物中分离出了艾瑞纳醇G-J和10种已知的麦角甾烷型甾醇。通过广泛的光谱分析，包括一维和二维核磁共振实验以及高分辨电喷雾电离质谱分析，并与先前报道的数据进行比较，阐明了它们的化学结构。在脂多糖（LPS）刺激的小鼠RAW264.7巨噬细胞中，根据对肿瘤坏死因子α（TNF-α）和一氧化氮（NO）产生的抑制作用，评估了分离出的化合物的抗炎效果。结果表明，艾瑞纳醇H和J以及2种麦角甾烷型甾醇对TNF-α分泌具有抑制活性，在10 μM时抑制值范围为33.7%至43.3%。艾瑞纳醇J和3种麦角甾烷型甾醇对NO产生具有显著抑制作用，在10 μM时抑制值范围为38.4%至71.5%。
