Research and Development Center, Nagaoka Co., Ltd., Ibaraki, Osaka 567-0005, Japan.
Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
Molecules. 2021 Dec 26;27(1):122. doi: 10.3390/molecules27010122.
Inhibition of fructose absorption may suppress adiposity and adiposity-related diseases caused by fructose ingestion. Eucalyptus leaf extract (ELE) inhibits intestinal fructose absorption (but not glucose absorption); however, its active compound has not yet been identified. Therefore, we evaluated the inhibitory activity of ELE obtained from using an intestinal fructose permeation assay with the human intestinal epithelial cell line Caco-2. The luminal sides of a cell monolayer model cultured on membrane filters were exposed to fructose with or without the ELE. Cellular fructose permeation was evaluated by measuring the fructose concentration in the medium on the basolateral side. ELE inhibited 65% of fructose absorption at a final concentration of 1 mg/mL. Oenothein B isolated from the ELE strongly inhibited fructose absorption; the inhibition rate was 63% at a final concentration of 5 μg/mL. Oenothein B did not affect glucose absorption. In contrast, the other major constituents (i.e., gallic acid and ellagic acid) showed little fructose-inhibitory activity. To our knowledge, this is the first report that oenothein B in ELE strongly inhibits fructose absorption in vitro. ELE containing oenothein B can prevent and ameliorate obesity and other diseases caused by dietary fructose consumption.
抑制果糖吸收可能抑制因摄入果糖而引起的肥胖和肥胖相关疾病。桉树叶提取物（ELE）抑制肠道果糖吸收（但不抑制葡萄糖吸收）；然而，其活性化合物尚未确定。因此，我们使用人肠上皮细胞系 Caco-2 进行的肠道果糖渗透测定法评估了从桉树中获得的 ELE 的抑制活性。在膜过滤器上培养的细胞单层模型的腔侧暴露于含有或不含有 ELE 的果糖。通过测量基底外侧侧介质中果糖的浓度来评估细胞内果糖的渗透。ELE 在最终浓度为 1mg/mL 时抑制 65%的果糖吸收。从 ELE 中分离出的奥尼汀 B 强烈抑制果糖吸收；在最终浓度为 5μg/mL 时，抑制率为 63%。奥尼汀 B 不影响葡萄糖吸收。相比之下，其他主要成分（即没食子酸和鞣花酸）表现出很少的果糖抑制活性。据我们所知，这是第一个报告表明 ELE 中的奥尼汀 B 强烈抑制体外果糖吸收的报告。含有奥尼汀 B 的 ELE 可以预防和改善因饮食中果糖摄入而引起的肥胖和其他疾病。
