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从……获得的纤维素酶混合物中主要酶在不同类型木质素上吸附的比较评价 。（原文中“from”后面缺少具体内容）

Comparative Evaluation of Adsorption of Major Enzymes in a Cellulase Cocktail Obtained from onto Different Types of Lignin.

作者信息

Lee Dae-Seok, Song Younho, Lee Yoon-Gyo, Bae Hyeun-Jong

机构信息

Bio-Energy Research Center, Chonnam National University, Gwangju 500-575, Korea.

Department of Wood Science and Landscape Architecture, Chonnam National University, Gwangju 500-757, Korea.

出版信息

Polymers (Basel). 2022 Jan 1;14(1):167. doi: 10.3390/polym14010167.

DOI:10.3390/polym14010167
PMID:35012188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8747337/
Abstract

Cellulase adsorption onto lignin decreases the productivity of enzymatic hydrolysis of lignocellulosic biomass. Here, adsorption of enzymes onto different types of lignin was investigated, and the five major enzymes-cellobiohydrolases (CBHs), endoglucanase (Cel7B), β-glucosidase (Cel3A), xylanase (XYNIV), and mannanase (Man5A)-in a cellulase cocktail obtained from were individually analyzed through SDS-PAGE and zymogram assay. Lignin was isolated from woody (oak and pine lignin) and herbaceous (rice straw and kenaf lignin) plants. The relative adsorption of CBHs compared to the control was in the range of 14.15-18.61%. The carbohydrate binding motif (CBM) of the CBHs contributed to higher adsorption levels in oak and kenaf lignin, compared to those in pine and rice lignin. The adsorption of endoglucanase (Cel7B) by herbaceous plant lignin was two times higher than that of woody lignin, whereas XYNIV showed the opposite pattern. β-glucosidase (Cel3A) displayed the highest and lowest adsorption ratios on rice straw and kenaf lignin, respectively. Mannanase (Man5A) was found to have the lowest adsorption ratio on pine lignin. Our results showed that the hydrophobic properties of CBM and the enzyme structures are key factors in adsorption onto lignin, whereas the properties of specific lignin types indirectly affect adsorption.

摘要

纤维素酶吸附到木质素上会降低木质纤维素生物质酶解的效率。在此，研究了酶在不同类型木质素上的吸附情况，并通过SDS - PAGE和酶谱分析对从[具体来源未给出]获得的纤维素酶混合物中的五种主要酶——纤维二糖水解酶（CBHs）、内切葡聚糖酶（Cel7B）、β - 葡萄糖苷酶（Cel3A）、木聚糖酶（XYNIV）和甘露聚糖酶（Man5A）进行了单独分析。木质素从木本植物（橡木和松木木质素）和草本植物（稻草和红麻木质素）中分离得到。与对照相比，CBHs的相对吸附率在14.15% - 18.61%范围内。与松木和稻草木质素相比，CBHs的碳水化合物结合基序（CBM）导致在橡木和红麻木质素中的吸附水平更高。草本植物木质素对内切葡聚糖酶（Cel7B）的吸附是木本木质素的两倍，而XYNIV则呈现相反的模式。β - 葡萄糖苷酶（Cel3A）在稻草和红麻木质素上分别表现出最高和最低的吸附率。发现甘露聚糖酶（Man5A）在松木木质素上的吸附率最低。我们的结果表明，CBM的疏水特性和酶结构是吸附到木质素上的关键因素，而特定木质素类型的特性间接影响吸附。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/a9a24595cbe8/polymers-14-00167-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/439ff595043a/polymers-14-00167-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/6090a9d4e8e4/polymers-14-00167-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/f7379e50dbd9/polymers-14-00167-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/b845e8f990a5/polymers-14-00167-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/8181f889517a/polymers-14-00167-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/a9a24595cbe8/polymers-14-00167-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/439ff595043a/polymers-14-00167-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/6090a9d4e8e4/polymers-14-00167-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/f7379e50dbd9/polymers-14-00167-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/b845e8f990a5/polymers-14-00167-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/8181f889517a/polymers-14-00167-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0cb/8747337/a9a24595cbe8/polymers-14-00167-g006.jpg

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