Anatomy and Pathology Department, Nanchang Medical College, Nanchang, Jiangxi, China.
School of Clinical Medicine, Nanchang University, Nanchang, Jiangxi, China.
Biol Direct. 2022 Jan 10;17(1):4. doi: 10.1186/s13062-021-00314-6.
Cervical cancer (CC) is one of the most common malignancies affecting female worldwide. Long non-coding RNAs (lncRNAs) are increasingly indicated as crucial participants and promising therapeutic targets in human cancers. The main objective of this study was to explore the functions and mechanism of LINC00885 in CC.
RT-qPCR and western blot were used to detect RNA and protein levels. Functional and mechanism assays were respectively done for the analysis of cell behaviors and molecular interplays.
Long intergenic non-coding RNA 885 (LINC00885) was discovered to be upregulated in CC tissues and cell lines through bioinformatics analysis and RT-qPCR. Overexpression of LINC00885 promoted proliferation and inhibited apoptosis, whereas its silence exerted opposite effects. The cytoplasmic localization of LINC00885 was ascertained and furthermore, LINC00885 competitively bound with miR-3150b-3p to upregulate BAZ2A expression in CC cells. Rescue assays confirmed that LINC00885 regulated CC proliferation and apoptosis through miR-3150b-3p/BAZ2A axis. Finally, we confirmed that LINC00885 aggravated tumor growth through animal experiments.
LINC00885 exerted oncogenic function in CC via regulating miR-3150b-3p/BAZ2A axis. These findings suggested LINC00885 might serve as a potential promising therapeutic target for CC patients.
宫颈癌（CC）是全球女性最常见的恶性肿瘤之一。长链非编码 RNA（lncRNA）越来越被认为是人类癌症中重要的参与者和有前途的治疗靶点。本研究的主要目的是探讨 LINC00885 在 CC 中的功能和机制。
使用 RT-qPCR 和 Western blot 检测 RNA 和蛋白水平。分别进行功能和机制测定，以分析细胞行为和分子相互作用。
通过生物信息学分析和 RT-qPCR 发现，长基因间非编码 RNA 885（LINC00885）在 CC 组织和细胞系中上调。LINC00885 的过表达促进增殖并抑制凋亡，而其沉默则产生相反的效果。确定了 LINC00885 的细胞质定位，并且 LINC00885 进一步与 miR-3150b-3p 竞争性结合，以在 CC 细胞中上调 BAZ2A 表达。挽救实验证实，LINC00885 通过 miR-3150b-3p/BAZ2A 轴调节 CC 增殖和凋亡。最后，我们通过动物实验证实 LINC00885 加重了肿瘤生长。
LINC00885 通过调节 miR-3150b-3p/BAZ2A 轴在 CC 中发挥致癌作用。这些发现表明，LINC00885 可能成为 CC 患者潜在的有前途的治疗靶点。
