Charley B, Fradelizi D
INRA, Station de Recherches de Virologie et d'Immunologie, Thiverval-Grignon, France.
Ann Rech Vet. 1987;18(3):227-32.
The in vitro effects of human or porcine Interleukin 2 (IL2) on Natural Killing (NK) activity were studied with blood lymphocytes of newborn piglets and of adult pigs. Large volumes of porcine IL2 were prepared by PHA stimulation of irradiated mesenteric lymph node cells and, following purification by gel-filtration chromatography, the apparent molecular weight of IL2 was 15,000 Da. Purified human and porcine IL2 as well as recombinant human IL2 were found to increase markedly NK activity of lymphocytes derived from adult animals. However, although newborn piglets-derived lymphocytes are sensitive to crude porcine IL2 supernatants (and to Interferon alpha as previously shown) their low NK activity is unaffected by purified IL2. These data suggest therefore the existence of differences in the ontogenic development of the lymphokines responsiveness of porcine NK cells.
用人或猪白细胞介素2（IL2）对新生仔猪和成年猪的血液淋巴细胞的自然杀伤（NK）活性进行了体外研究。通过PHA刺激经辐照的肠系膜淋巴结细胞制备了大量猪IL2，经凝胶过滤层析纯化后，IL2的表观分子量为15,000道尔顿。发现纯化的人IL2、猪IL2以及重组人IL2能显著增强成年动物淋巴细胞的NK活性。然而，尽管新生仔猪来源的淋巴细胞对粗制猪IL2上清液敏感（如先前所示，对α干扰素也敏感），但其较低的NK活性不受纯化IL2的影响。因此，这些数据表明猪NK细胞的淋巴因子反应性在个体发育过程中存在差异。
