Liu S, Wen Y, Quan B, Lin J, Zhu Z, Tang J, Han S
Department of General Surgery, Pingxiang People's Hospital, Pingxiang 337000, China.
Department of Oncology, Pingxiang People's Hospital, Pingxiang 337000, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Dec 20;41(12):1885-1891. doi: 10.12122/j.issn.1673-4254.2021.12.19.
To investigate the expression of miR-3682-3p in hepatocellular carcinoma (HCC) and its correlation with clinical parameters and prognosis of HCC.
We conducted a bioinformatics analysis of the expression of miR-3682-3p in HCC and its correlation with the patients' survival, and examined its expression in 18 pairs of fresh and 90 pairs of paraffin-embedded HCC and adjacent tissues using real-time fluorescence quantitative PCR and in situ hybridization, respectively. The correlation of miR-3682-3p expression in HCC with the clinical parameters and prognosis of the patients was analyzed. Multivariate regression analysis was used to explore the possibility of miR-3682-3p expression as an independent prognostic factor of HCC.
Bioinformatics analysis showed that miR-3682-3p was highly expressed in HCC and significantly correlated with the survival time of HCC patients (χ=8.793, < 0.001). The expression of miR-3682-3p was significantly up-regulated in fresh HCC tissues as compared with the adjacent liver tissues (=3.073, =0.007). In paraffin-embedded samples, in situ hybridization revealed positive miR-3682-3p expression in the cytoplasm of HCC and adjacent tissues, and its expression was signifcantly up-regulated in HCC tissues (=2.659, =0.009). The expression level of miR-3682-3p was significantly correlated with American Joint Commission on Cancer (AJCC; 8th edition) stage (χ=4.272, = 0.039), HBV surface antigen status (χ=5.143, =0.023), recurrence (χ=4.593, =0.032), tumor size (χ=4.580, =0.032) and Edmondson Steiner grade (χ=4.068, =0.044). Kaplan-Meier analysis showed that a higher expression of miR-3682-3p was associated with a shorter overall survival time (χ=4.169, =0.041) and disease-free survival time (χ=4.078, =0.043) of the patients. Multivariate analysis suggested that miR-3682-3p expression was an independent predictor of the prognosis of HCC patients.
MiR-3682-3p is up-regulated in HCC to serve as a significant factor that contributes to the occurrence and a poor prognosis of HCC.
探讨miR - 3682 - 3p在肝细胞癌（HCC）中的表达及其与HCC临床参数和预后的相关性。
我们对miR - 3682 - 3p在HCC中的表达及其与患者生存的相关性进行了生物信息学分析，并分别使用实时荧光定量PCR和原位杂交检测了其在18对新鲜HCC及90对石蜡包埋的HCC及其癌旁组织中的表达。分析HCC中miR - 3682 - 3p表达与患者临床参数和预后的相关性。采用多因素回归分析探讨miR - 3682 - 3p表达作为HCC独立预后因素的可能性。
生物信息学分析显示，miR - 3682 - 3p在HCC中高表达，且与HCC患者的生存时间显著相关（χ=8.793，P<0.001）。与癌旁肝组织相比，新鲜HCC组织中miR - 3682 - 3p的表达显著上调（t=3.073，P=0.007）。在石蜡包埋样本中，原位杂交显示miR - 3682 - 3p在HCC及癌旁组织的细胞质中呈阳性表达，且其在HCC组织中的表达显著上调（t=2.659，P=0.009）。miR - 3682 - 3p的表达水平与美国癌症联合委员会（AJCC；第8版）分期（χ=4.272，P=0.039）、乙肝表面抗原状态（χ=5.143，P=0.023）、复发（χ=4.593，P=0.032）、肿瘤大小（χ=4.580，P=0.032）及Edmondson Steiner分级（χ=4.068，P=0.044）显著相关。Kaplan - Meier分析显示，miR -
