马的 DNA 甲基化衰老和转录组研究。
DNA methylation aging and transcriptomic studies in horses.
机构信息
Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA.
出版信息
Nat Commun. 2022 Jan 10;13(1):40. doi: 10.1038/s41467-021-27754-y.
Cytosine methylation patterns have not yet been thoroughly studied in horses. Here, we profile n = 333 samples from 42 horse tissue types at loci that are highly conserved between mammalian species using a custom array (HorvathMammalMethylChip40). Using the blood and liver tissues from horses, we develop five epigenetic aging clocks: a multi-tissue clock, a blood clock, a liver clock and two dual-species clocks that apply to both horses and humans. In addition, using blood methylation data from three additional equid species (plains zebra, Grevy's zebras and Somali asses), we develop another clock that applies across all equid species. Castration does not significantly impact the epigenetic aging rate of blood or liver samples from horses. Methylation and RNA data from the same tissues define the relationship between methylation and RNA expression across horse tissues. We expect that the multi-tissue atlas will become a valuable resource.
马的胞嘧啶甲基化模式尚未得到充分研究。在这里，我们使用定制的芯片（HorvathMammalMethylChip40）在 42 种马组织类型的 n=333 个样本中，对在哺乳动物物种之间高度保守的基因座进行了分析。我们利用马的血液和肝脏组织，开发了五个表观遗传衰老时钟：一个多组织时钟、一个血液时钟、一个肝脏时钟和两个适用于马和人类的双物种时钟。此外，我们还利用来自另外三种马科动物（平原斑马、格雷维斑马和索马里驴）的血液甲基化数据，开发了另一个适用于所有马科动物的时钟。去势对马的血液或肝脏样本的表观遗传衰老率没有显著影响。来自相同组织的甲基化和 RNA 数据定义了马组织中甲基化和 RNA 表达之间的关系。我们预计多组织图谱将成为一个有价值的资源。
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