Rikkers Margot, Korpershoek Jasmijn V, Levato Riccardo, Malda Jos, Vonk Lucienne A
Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
NPJ Regen Med. 2022 Jan 10;7(1):2. doi: 10.1038/s41536-021-00203-6.
Over the past two decades, evidence has emerged for the existence of a distinct population of endogenous progenitor cells in adult articular cartilage, predominantly referred to as articular cartilage-derived progenitor cells (ACPCs). This progenitor population can be isolated from articular cartilage of a broad range of species, including human, equine, and bovine cartilage. In vitro, ACPCs possess mesenchymal stromal cell (MSC)-like characteristics, such as colony forming potential, extensive proliferation, and multilineage potential. Contrary to bone marrow-derived MSCs, ACPCs exhibit no signs of hypertrophic differentiation and therefore hold potential for cartilage repair. As no unique cell marker or marker set has been established to specifically identify ACPCs, isolation and characterization protocols vary greatly. This systematic review summarizes the state-of-the-art research on this promising cell type for use in cartilage repair therapies. It provides an overview of the available literature on endogenous progenitor cells in adult articular cartilage and specifically compares identification of these cell populations in healthy and osteoarthritic (OA) cartilage, isolation procedures, in vitro characterization, and advantages over other cell types used for cartilage repair. The methods for the systematic review were prospectively registered in PROSPERO (CRD42020184775).
在过去二十年中，有证据表明成年关节软骨中存在一类独特的内源性祖细胞群体，主要被称为关节软骨衍生祖细胞（ACPCs）。这种祖细胞群体可以从包括人类、马和牛软骨在内的多种物种的关节软骨中分离出来。在体外，ACPCs具有间充质基质细胞（MSC）样特征，如集落形成潜力、广泛增殖和多向分化潜能。与骨髓来源的MSCs不同，ACPCs没有肥大分化的迹象，因此具有软骨修复的潜力。由于尚未建立专门识别ACPCs的独特细胞标志物或标志物组，分离和鉴定方案差异很大。本系统综述总结了这种用于软骨修复治疗的有前景细胞类型的最新研究。它概述了有关成年关节软骨内源性祖细胞的现有文献，并特别比较了在健康和骨关节炎（OA）软骨中这些细胞群体的鉴定、分离程序、体外特征以及与用于软骨修复的其他细胞类型相比的优势。该系统综述的方法已在PROSPERO（CRD42020184775）中进行了前瞻性注册。
