Tian Jun, Dai Shao-Bing, Jiang Si-Si, Yang Wen-Yi, Yan Yi-Qun, Lin Zhi-Hao, Dong Jia-Xian, Liu Yi, Zheng Ran, Chen Ying, Zhang Bao-Rong, Pu Jia-Li
Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Department of Anesthesiology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, Zhejiang, China.
NPJ Parkinsons Dis. 2022 Jan 10;8(1):5. doi: 10.1038/s41531-021-00271-x.
Recent evidence suggests that innate and adaptive immunity play a crucial role in Parkinson's disease (PD). However, studies regarding specific immune cell classification in the peripheral blood in PD remain lacking. Therefore, we aimed to explore the different immune status in patients with PD at different ages of onset. We included 22 patients; among them were 10 who had early-onset PD (EOPD) and 12 had late-onset PD (LOPD) and 10 young healthy controls (YHCs) and 8 elder HCs (EHCs). Mass cytometry staining technology was used to perform accurate immunotyping of cell populations in the peripheral blood. Motor symptoms and cognitive function were assessed using the Unified Parkinson's Disease Rating Scale (UPDRS) III score and Mini-mental State Examination (MMSE) score, respectively. T test and ANOVA statistical analysis were performed on the frequency of annotated cell population. Linear regression model was used to analyze the correlation between clusters and clinical symptoms. We characterized 60 cell clusters and discovered that the immune signature of PD consists of cluster changes, including decreased effector CD8 T cells, lower cytotoxicity natural killer (NK) cells and increased activated monocytes in PD patients. In summary, we found that CD8 T cells, NK cells, and monocytes were associated with PD. Furthermore, there may be some differences in the immune status of patients with EOPD and LOPD, suggesting differences in the pathogenesis between these groups.
最近的证据表明，先天性免疫和适应性免疫在帕金森病（PD）中起着至关重要的作用。然而，关于PD患者外周血中特定免疫细胞分类的研究仍然缺乏。因此，我们旨在探讨不同发病年龄的PD患者的不同免疫状态。我们纳入了22名患者，其中10名是早发性PD（EOPD）患者，12名是晚发性PD（LOPD）患者，以及10名年轻健康对照者（YHCs）和8名老年健康对照者（EHCs）。采用质谱流式细胞术染色技术对外周血中的细胞群体进行精确的免疫分型。分别使用统一帕金森病评定量表（UPDRS）III评分和简易精神状态检查表（MMSE）评分评估运动症状和认知功能。对注释细胞群体的频率进行t检验和方差分析统计分析。使用线性回归模型分析细胞簇与临床症状之间的相关性。我们对60个细胞簇进行了特征描述，发现PD的免疫特征包括细胞簇变化，包括PD患者中效应性CD8 T细胞减少、细胞毒性自然杀伤（NK）细胞降低以及活化单核细胞增加。总之，我们发现CD8 T细胞、NK细胞和单核细胞与PD有关。此外，EOPD和LOPD患者的免疫状态可能存在一些差异，表明这些组之间发病机制的差异。
