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从巴西橡胶树种子残渣中评估体外抗氧化提取物。

In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed.

机构信息

Chemistry Department, Federal University of Fronteira Sul, Realeza, Paraná, 85770-000, Brazil.

Chemistry Department, Federal University of Paraná, Curitiba, Paraná, 81531-990, Brazil.

出版信息

Sci Rep. 2022 Jan 10;12(1):480. doi: 10.1038/s41598-021-04017-w.

Abstract

The antioxidants used in the food industry are essential to inhibit the formation of free radicals, preserving the existing properties in the different matrices. However, the insecurity of the synthetic antioxidants regarding human health propels search for natural substrates with potential antioxidant activity as an alternative to synthetic compounds. In this way, the work had as objective obtaining extracts from the seed pomace of the Hevea brasiliensis (rubber tree), relating the contents of flavonoids and total phenols in the application as an antioxidant. The methodology consisted of the extraction using four solvents, varying extractive methods, time, and seed concentrations. The antioxidant activity in vitro was evaluated by capturing the DPPH (2,2-diphenyl-1-picryl-hydrazil) radical. The optimized results demonstrate that the aqueous extracts produced in the Soxhlet in the concentrations of 85 g L and retention time of 4 h reached 37.73 ± 1.69% in the antioxidant tests of the free radical DPPH capture, 1405.15 mg EAC 100 g in the quantification of phenolic compounds and 223.34 mg 100 g of total flavonoids. Thus, this work may contribute to the realization of studies and future research for characterization and identification concerning which phenolic compounds and flavonoids attribute the antioxidant characteristic to the extracts produced, enabling the discovery of products with high added value in the production chain. In addition, because the water used as a solvent showed greater antioxidant potential between the extracts, the non-toxic and environmentally friendly character is highlighted, allowing a wide variety of applications in the food industry.

摘要

抗氧化剂在食品工业中是必不可少的,它可以抑制自由基的形成,保持不同基质中现有物质的特性。然而,由于合成抗氧化剂对人类健康的安全性存在隐患,因此人们正在寻找具有潜在抗氧化活性的天然物质作为合成化合物的替代品。在这种情况下,本研究的目的是从巴西橡胶树(Hevea brasiliensis)的种子渣中获得提取物,探讨其在应用中的类黄酮和总酚含量作为抗氧化剂的效果。该方法使用了四种溶剂,通过改变提取方法、时间和种子浓度来提取。体外抗氧化活性通过捕获 DPPH(2,2-二苯基-1-苦基肼)自由基来评估。优化结果表明,在索氏提取器中以 85 g/L 的浓度提取 4 小时,得到的水提取物在自由基 DPPH 捕获的抗氧化试验中达到 37.73±1.69%的效果,在测定酚类化合物时达到 1405.15 mg EAC/100 g,总类黄酮达到 223.34 mg/100 g。因此,这项工作可能有助于对生产过程中产生的提取物的特征和鉴定进行研究和未来研究,发现具有高附加值的产品。此外,由于作为溶剂的水在提取物之间表现出更大的抗氧化潜力,因此突出了其无毒和环保的特点,允许在食品工业中进行广泛的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c50b/8748519/f908fe6b404e/41598_2021_4017_Fig1_HTML.jpg

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