Liaoning Ocean and Fisheries Science Research Institute, 50 Heishijiao St., Dalian, 116023, Liaoning, China.
Sci Rep. 2022 Jan 10;12(1):471. doi: 10.1038/s41598-021-04431-0.
R. esculentum is a popular seafood in Asian countries and an economic marine fishery resource in China. However, the genetic linkage map and growth-related molecular markers are still lacking, hindering marker assisted selection (MAS) for genetic improvement of R. esculentum. Therefore, we firstly used 2b-restriction site-associated DNA (2b-RAD) method to sequence 152 R. esculentum specimens and obtained 9100 single nucleotide polymorphism (SNP) markers. A 1456.34 cM linkage map was constructed using 2508 SNP markers with an average interval of 0.58 cM. Then, six quantitative trait loci (QTLs) for umbrella diameter and body weight were detected by QTL analysis based on the new linkage map. The six QTLs are located on four linkage groups (LGs), LG4, LG13, LG14 and LG15, explaining 9.4% to 13.4% of the phenotypic variation. Finally, 27 candidate genes in QTLs regions of LG 14 and 15 were found associated with growth and one gene named RE13670 (sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1-like) may play an important role in controlling the growth of R. esculentum. This study provides valuable information for investigating the growth mechanism and MAS breeding in R. esculentum.
滑菇是亚洲国家常见的海鲜，也是中国重要的海洋渔业资源。然而，其遗传连锁图谱和生长相关的分子标记仍不完善，阻碍了利用标记辅助选择（MAS）进行遗传改良。因此，我们首次使用 2b 限制酶相关 DNA（2b-RAD）方法对 152 个滑菇样本进行测序，获得了 9100 个单核苷酸多态性（SNP）标记。利用 2508 个 SNP 标记构建了一个 1456.34cM 的连锁图谱，平均标记间隔为 0.58cM。然后，基于新的连锁图谱，通过 QTL 分析检测到了伞径和体重的 6 个数量性状位点（QTLs）。这 6 个 QTL 位于 4 个连锁群（LGs）上，LG4、LG13、LG14 和 LG15，分别解释了 9.4%到 13.4%的表型变异。最后，在 LG14 和 LG15 的 QTL 区域发现了 27 个与生长相关的候选基因，其中一个名为 RE13670（ sushi、von Willebrand 因子 A、EGF 和 pentraxin 结构域蛋白 1 样）的基因可能在控制滑菇生长中发挥重要作用。本研究为研究滑菇生长机制和 MAS 育种提供了有价值的信息。
