Metcalf D, Roberts T M, Cherington V, Dunn A R
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
EMBO J. 1987 Dec 1;6(12):3703-9. doi: 10.1002/j.1460-2075.1987.tb02704.x.
A retrovirus encoding polyoma middle T antigen has been used to infect a murine hemopoietic cell line (FDC-P1) dependent on either granulocyte-macrophage colony-stimulating factor (GM-CSF) or multipotential colony-stimulating factor (Multi-CSF). A number of cell lines have been established on the basis of their initial ability to proliferate in the absence of added colony-stimulating factor (CSF). The transformed lines display one of three patterns of growth in vitro: those able to grow fully autonomously; those whose proliferation depends on cell density; and those displaying dependence on added CSF regardless cell density. This latter class of cells are reminiscent of the majority of primary myeloid leukemic cells. Unlike parental FDC-P1 cells, all three classes of transformed cells are leukemogenic in syngeneic mice; moreover, they produce variable amounts of GM-CSF which we believe underlies their neoplastic behavior.
一种编码多瘤病毒中T抗原的逆转录病毒已被用于感染依赖粒细胞-巨噬细胞集落刺激因子（GM-CSF）或多能集落刺激因子（Multi-CSF）的小鼠造血细胞系（FDC-P1）。基于它们在不添加集落刺激因子（CSF）的情况下最初的增殖能力，已经建立了许多细胞系。这些转化细胞系在体外呈现出三种生长模式之一：能够完全自主生长的；其增殖依赖于细胞密度的；以及无论细胞密度如何都依赖于添加的CSF的。后一类细胞让人联想到大多数原发性髓系白血病细胞。与亲代FDC-P1细胞不同，所有这三类转化细胞在同基因小鼠中都具有致白血病性；此外，它们产生不同量的GM-CSF，我们认为这是它们肿瘤行为的基础。
