Adkins B, Leutz A, Graf T
Cell. 1984 Dec;39(3 Pt 2):439-45. doi: 10.1016/0092-8674(84)90451-3.
Chicken myeloid cells transformed by the v-myb-or v-myc-containing leukemia viruses, E26 and OK 10, respectively, require chicken myelomonocytic growth factor (cMGF) for proliferation in vitro. Upon superinfection with retroviruses carrying oncogenes of the src gene family, these myeloid cells acquire the ability to grow in the absence of exogenous cMGF. Conditioned medium prepared from superinfected E26 cells contains a growth-stimulating activity similar in biological and immunological properties to cMGF. This activity is reduced by more than 80% following absorption of conditioned media with antiserum against cMGF. Incubation of superinfected E26 cells with an immunoglobulin fraction of antiserum against cMGF inhibits their proliferation, indicating that the cells are dependent on the secreted factor. We conclude that viral oncogenes of the src family can induce chicken myeloid cells to produce a cMGF-like factor(s) that stimulates proliferation of these cells in an autocrine fashion.
分别由含v-myb或v-myc的白血病病毒E26和OK 10转化的鸡髓细胞，在体外增殖需要鸡髓单核细胞生长因子（cMGF）。在用携带src基因家族癌基因的逆转录病毒进行超感染后，这些髓细胞获得了在无外源性cMGF的情况下生长的能力。从超感染的E26细胞制备的条件培养基含有一种生长刺激活性，其生物学和免疫学特性与cMGF相似。用抗cMGF抗血清吸收条件培养基后，这种活性降低了80%以上。用抗cMGF抗血清的免疫球蛋白部分孵育超感染的E26细胞可抑制其增殖，表明这些细胞依赖于分泌因子。我们得出结论，src家族的病毒癌基因可诱导鸡髓细胞产生一种cMGF样因子，以自分泌方式刺激这些细胞的增殖。
