Hapel A J, Warren H S, Hume D A
Blood. 1984 Oct;64(4):786-90.
The cell lines FDC-Pl and 32D cl-23 have previously been used as unique indicators for the growth-promoting activity of interleukin-3. We show that FDC-Pl cells respond to granulocyte/macrophage colony-stimulating factor (GM-CSF, CSF-2) as well as to interleukin-3. In keeping with this finding, FDC-Pl cells express the macrophage-specific marker, F4/80. FDC-Pl cells do not, however, respond to macrophage CSF (M-CSF, CSF-1). In contrast, 32D cl-23 cells do not respond to GM-CSF and lack F4/80. Instead, 32D cl-23 cells respond to an as yet undefined factor in conditioned medium (CM) from the primate T cell line, MLA-144, and CM from mitogen-stimulated human lymphocytes (HLCM). 32D cl-23 cells are Lyt-1+. Both FDC-Pl and 32D cl-23 cells consume interleukin-3, but only FDC Pl cells consume GM-CSF. Similarly, 32D cl-23, but not FDC-Pl, cells consume 32D cl-23 growth factor from MLA-144 CM and HLCM. Interleukin-3-dependent cell lines must therefore concurrently express different functional cell surface receptors for a variety of biochemically distinct growth factors.
细胞系FDC-Pl和32D cl-23先前已被用作白细胞介素-3促生长活性的独特指标。我们发现FDC-Pl细胞对粒细胞/巨噬细胞集落刺激因子（GM-CSF，CSF-2）以及白细胞介素-3均有反应。与这一发现一致的是，FDC-Pl细胞表达巨噬细胞特异性标志物F4/80。然而，FDC-Pl细胞对巨噬细胞集落刺激因子（M-CSF，CSF-1）无反应。相反，32D cl-23细胞对GM-CSF无反应且缺乏F4/80。取而代之的是，32D cl-23细胞对来自灵长类T细胞系MLA-144的条件培养基（CM）以及有丝分裂原刺激的人淋巴细胞的CM（HLCM）中的一种尚未明确的因子有反应。32D cl-23细胞是Lyt-1+。FDC-Pl和32D cl-23细胞均消耗白细胞介素-3，但只有FDC-Pl细胞消耗GM-CSF。同样，32D cl-23细胞而非FDC-Pl细胞消耗来自MLA-144 CM和HLCM的32D cl-23生长因子。因此，白细胞介素-3依赖的细胞系必须同时表达针对多种生化性质不同的生长因子的不同功能性细胞表面受体。
