School of Public Health, Jilin University, Changchun 130021, China.
ACS Appl Bio Mater. 2021 Jan 18;4(1):420-427. doi: 10.1021/acsabm.0c00897. Epub 2020 Dec 15.
The effective identification and quantitative determination of is a major public health concern. Here, an innovative strategy that combines a bacteria-imprinted polydimethylsiloxane film for bacterial recognition and fluorescence resonance energy transfer platform for turn-on fluorescence sensing is demonstrated. The bacteria-imprinted polydimethylsiloxane film was facilely fabricated to generate corresponding specific sites on the polydimethylsiloxane surface via stamp imprinting using as template followed by modification with 1H,1H,2H,2H-perfluorooctyltriethoxysilane. The fluorescence resonance energy transfer platform was developed through electrostatic interaction between citrate-functional copper clusters and dopamine-stabilized gold nanoparticles. When the are present, the 1H,1H,2H,2H-perfluorooctyltriethoxysilane-modified bacteria-imprinted polydimethylsiloxane film can precisely capture the target; subsequently, the negatively charged bacteria compete with citrate-functional copper clusters and bind to dopamine-stabilized gold nanoparticles, leading to the fluorescence recovery of citrate-functional copper clusters. The entire detection process was achieved within 135 min, showing a wide linear calibration response from 10 to 1 × 10 cfu mL with a low detection limit of 11.12 cfu mL. Furthermore, the recoveries from spiked samples were from 97.7 to 101.90% with relative standard derivations lower than 10%. The established label-free assay of measuring is rapid, sensitive, specific, and efficient.
有效识别和定量测定 是一个主要的公共卫生关注点。在这里，展示了一种将细菌印迹聚二甲基硅氧烷膜用于细菌识别和荧光共振能量转移平台用于开启荧光传感的创新策略。通过使用 作为模板，通过压印在聚二甲基硅氧烷表面上生成相应的特定位点，然后用 1H、1H、2H、2H-全氟辛基三乙氧基硅烷进行修饰，制备了细菌印迹聚二甲基硅氧烷膜。荧光共振能量转移平台是通过柠檬酸功能化铜簇和多巴胺稳定的金纳米颗粒之间的静电相互作用开发的。当 存在时，1H、1H、2H、2H-全氟辛基三乙氧基硅烷修饰的细菌印迹聚二甲基硅氧烷膜可以精确捕获靶标；随后，带负电荷的细菌与柠檬酸功能化铜簇竞争并与多巴胺稳定的金纳米颗粒结合，导致柠檬酸功能化铜簇的荧光恢复。整个检测过程在 135 分钟内完成，表现出从 10 到 1×10 cfu mL 的宽线性校准响应，检测限低至 11.12 cfu mL。此外，从加标样品中的回收率为 97.7%至 101.90%，相对标准偏差低于 10%。建立的用于测量 的无标记测定法快速、灵敏、特异、高效。
