Department of Food Science and Technology, University of California, Davis, California 95616, United States.
School of Chemical Sciences, The University of Auckland, Auckland 1010, New Zealand.
ACS Appl Bio Mater. 2021 Jan 18;4(1):514-522. doi: 10.1021/acsabm.0c01051. Epub 2020 Dec 29.
Efficient inactivation and removal of pathogenic biofilms in food and biomedical environments remain a significant challenge for food safety applications and medical facilities. This research aims to develop food-grade microcarriers for the targeted delivery of a photosensitizer, curcumin, and photodynamic inactivation of a model pathogenic bacterial biofilm. The microcarriers evaluated in this study include alive yeast cell carriers, deactivated yeast cell carriers, and yeast cell wall particles. The microcarriers were evaluated based on the encapsulation yield of a model photosensitizer (curcumin), binding of the microcarriers to biofilms, and inactivation of the bacteria in the biofilms. The results illustrate that the combination of binding affinity, encapsulation yield, and the intracellular composition of the microcarriers influenced the overall inactivation of bacteria in the biofilms. All of the selected compositions achieved more than 93% inactivation of the bacteria in the biofilm using the photodynamic treatment, and the yeast cell wall particles with curcumin achieved over 99% inactivation of the bacteria in the biofilm matrix. In addition, all of the selected compositions demonstrated significant potential to remove the biofilm from the plastic surface, suggesting the role of binding affinity of the microcarriers in removal of the biofilm from surfaces. Overall, this study developed biomaterial formulations for targeted photodynamic inactivation and potential removal of biofilms.
在食品安全应用和医疗设施中，高效灭活和去除食品和生物医学环境中的致病生物膜仍然是一个重大挑战。本研究旨在开发食品级微载体，用于靶向递送光敏剂姜黄素，并进行光动力灭活模型致病细菌生物膜。本研究评估的微载体包括活酵母细胞载体、失活酵母细胞载体和酵母细胞壁颗粒。根据模型光敏剂（姜黄素）的包封产率、微载体与生物膜的结合以及生物膜中细菌的失活情况对微载体进行了评估。结果表明，结合亲和力、包封产率和微载体的细胞内成分影响生物膜中细菌的整体失活。所有选定的组合物在光动力处理下都能实现生物膜中细菌 93%以上的失活，而载有姜黄素的酵母细胞壁颗粒能实现生物膜基质中 99%以上的细菌失活。此外，所有选定的组合物都表现出从塑料表面有效去除生物膜的巨大潜力，表明微载体的结合亲和力在生物膜从表面去除中的作用。总的来说，本研究开发了用于靶向光动力灭活和潜在去除生物膜的生物材料配方。
