Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, Lund 221 00, Sweden.
Key Laboratory of Interface Science and Engineering in Advanced materials, Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2829-2838. doi: 10.1021/acsabm.1c00045. Epub 2021 Feb 23.
After the emergence of multidrug-resistant strains, antibiotic resistance in bacteria has become an important problem. Thus, materials for combating multidrug-resistant bacteria are of vital importance. In this work, we developed an antibacterial material that can selectively capture and destruct bacteria on the basis of their physical characteristics. To achieve bacterial capture and deactivation with a single material, we used bacterial cells as templates to synthesize surface-imprinted polymer beads in bacteria-stabilized Pickering emulsions. Acrylate-functionalized polyethylenimine was used to coat the bacterial surface so that the coated bacteria can act as a particle stabilizer to establish an oil-in-water Pickering emulsion. Hydrophobic Ag nanoparticles were introduced into the oil phase composed of cross-linking monomers. Bacteria-imprinted beads (BIB) were obtained after the oil phase was polymerized. Bacterial binding experiments confirmed the importance of the imprinted sites for specific recognition with the target bacteria. The Ag nanoparticles embedded inside the polymer beads enhanced bacterial inactivation and reduced the leakage of heavy metal in aquatic environment. The combination of bacteria-imprinting with delivery of general-purpose antibacterial reagents offers a useful approach toward selective capture and destruction of bacteria.
随着多药耐药菌株的出现，细菌的抗生素耐药性已成为一个重要问题。因此，针对多药耐药菌的材料至关重要。在这项工作中，我们开发了一种基于物理特性选择性捕获和破坏细菌的抗菌材料。为了用单一材料实现细菌的捕获和失活，我们使用细菌细胞作为模板，在细菌稳定的 Pickering 乳液中合成表面印迹聚合物珠。使用丙烯酰胺功能化的聚乙烯亚胺对细菌表面进行涂层，以使涂层细菌可以作为颗粒稳定剂来建立油包水 Pickering 乳液。将疏水性 Ag 纳米颗粒引入由交联单体组成的油相。聚合油相后，得到细菌印迹珠（BIB）。细菌结合实验证实了印迹部位对于与目标细菌进行特异性识别的重要性。嵌入聚合物珠内的 Ag 纳米颗粒增强了对细菌的失活作用，并减少了重金属在水环境中的泄漏。将细菌印迹与通用抗菌试剂的递送相结合，为选择性捕获和破坏细菌提供了一种有用的方法。
