Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, 637371 Singapore.
International Nanobody Research Centre of Guangxi, Guangxi Medical University, Nanning, Guangxi 530021, China.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2192-2216. doi: 10.1021/acsabm.0c01340. Epub 2021 Jan 12.
In situ self-assembly has attracted increasing research interest for applications in imaging and therapy in recent years. Particularly for protease-activated developments, inspiration is drawn from the innate specificity of their catalytic activities, rapid discovery of the various roles they play in the proliferation of certain diseases, and inherent susceptibility of small molecule peptide conjugates to proteolytic digestion in vivo. The overexpression of a disease-related protease of interest can be exploited as an endogenous stimulus for site-specific self-assembly to largely amplify a molecular event happening at the cellular level. This holds great potential for applications in early stage disease detection, long-term disease monitoring, and sustained therapeutic effects. This review summarizes the recent developments in protease-activated self-assemblies for imaging and therapeutic applications toward the manifestation of tumors, bacterial infections, neurodegenerative disorders, and wound recovery.
近年来，原位自组装因其在成像和治疗方面的应用而引起了越来越多的研究兴趣。特别是对于蛋白酶激活的发展，灵感来自于它们催化活性的固有特异性、快速发现它们在某些疾病增殖中所扮演的各种角色，以及小分子肽缀合物在体内对蛋白水解消化的固有敏感性。感兴趣的疾病相关蛋白酶的过表达可以被利用为原位特异性自组装的内源性刺激，从而大大放大发生在细胞水平的分子事件。这在早期疾病检测、长期疾病监测和持续治疗效果方面具有巨大的应用潜力。本文综述了近年来蛋白酶激活自组装在肿瘤、细菌感染、神经退行性疾病和伤口恢复等方面的成像和治疗应用的研究进展。
