Shandong Provincial Engineering Research Center of Novel Pharmaceutical Excipients and Controlled Release Preparations, School of Pharmacy, Dezhou University, Dezhou 253000, China.
Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
ACS Appl Mater Interfaces. 2024 Sep 4;16(35):45821-45829. doi: 10.1021/acsami.4c10653. Epub 2024 Aug 23.
In situ self-assembly in living systems is referred to as the processes that regulate assembly by stimuli-responsive reactions at target sites under physiological conditions. Due to the advantages of precisely forming well-defined nanostructures at pathological lesions, in situ-formed assemblies with tailored bioactivity are promising for the development of next-generation biomedical agents. In this Perspective, we summarize the progress of in situ self-assembly of peptides in living cells with an emphasis on the state-of-the-art strategies regulating assembly processes, establishing complexity within assembly systems, and exploiting their applications in biomedicines. We also provide our forward conceiving perspectives on the challenges in the development of in situ assembly in living cells to demonstrate its great potential in creating biomaterials for healthcare in the future.
在活系统中的原位自组装是指在生理条件下通过在靶位的刺激响应反应来调节组装的过程。由于在病理损伤处精确形成定义明确的纳米结构的优势，具有定制生物活性的原位形成的组装体有望成为下一代生物医学试剂的发展方向。在本观点中，我们总结了在活细胞中肽的原位自组装的进展，重点介绍了调节组装过程、在组装系统内建立复杂性以及将其应用于生物医学的最新策略。我们还提供了对活细胞中原位组装发展的挑战性的前瞻性设想，以展示其在未来创造医疗保健生物材料方面的巨大潜力。
