Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, P. R. China.
ACS Appl Bio Mater. 2021 Apr 19;4(4):3214-3223. doi: 10.1021/acsabm.0c01608. Epub 2021 Mar 11.
Macrophage cells are plastic and can be polarized into opposing phenotypes, pro-inflammatory (M1-like cells) or anti-inflammatory (M2-like cells). Reprograming of M2-like cells into M1 phenotype will contribute significantly to combatting cancer. Gold nanoparticles (AuNPs) are intensively studied in various fields for their distinctive photo-chemical properties. However, the immune response of AuNPs is still unclear. In this study, AuNPs and CaCO-encapsulated Au nanoparticles (Au@CaCO NPs) were synthesized as stimuli for macrophage modulation. Co-incubation of AuNPs and macrophages leads to a dramatically elongated macrophage cell morphology. Moreover, increased expression of M2 biomarker and M2-inducing cytokines suggests that AuNPs induce macrophage polarization toward M2 phenotype. More interestingly, the co-incubation of Au@CaCO NPs and macrophage cells resulted in a round cellular morphology and induced the secretion of M1 biomarker and inflammatory cytokines. Our studies demonstrate that the strategy of CaCO-encapsulated Au nanoparticles can be used in modulating the polarization of M1 macrophages. Our strategy provides an efficient method for activating inflammation in macrophages, which will be useful for the application of nanoparticles in cancer therapy.
巨噬细胞具有可塑性，可以被极化成为两种相反的表型，促炎型（M1 样细胞）或抗炎型（M2 样细胞）。将 M2 样细胞重编程为 M1 表型将显著有助于对抗癌症。金纳米粒子（AuNPs）因其独特的光化学性质而在各个领域得到了广泛的研究。然而，AuNPs 的免疫反应仍不清楚。在这项研究中，合成了金纳米粒子（AuNPs）和 CaCO 封装的金纳米粒子（Au@CaCO NPs）作为巨噬细胞调节的刺激物。AuNPs 与巨噬细胞共孵育会导致巨噬细胞形态显著伸长。此外，M2 生物标志物和 M2 诱导细胞因子的表达增加表明，AuNPs 诱导巨噬细胞向 M2 表型极化。更有趣的是，Au@CaCO NPs 与巨噬细胞共孵育导致细胞形态呈圆形，并诱导 M1 生物标志物和炎症细胞因子的分泌。我们的研究表明，CaCO 封装的金纳米粒子的策略可用于调节 M1 巨噬细胞的极化。我们的策略为激活巨噬细胞中的炎症提供了一种有效的方法，这将有助于纳米粒子在癌症治疗中的应用。
