College of Chemistry, Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Tianjin Normal University, Tianjin 300387, PR China.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
ACS Appl Bio Mater. 2021 Apr 19;4(4):3443-3452. doi: 10.1021/acsabm.1c00017. Epub 2021 Mar 10.
Nanomaterial-based mimetic enzymes, called nanozymes, received more and more attention in recent decades; however, their lack of biocompatibility limited the biomedical applications, which could be solved by surface modification. In this work, the CoO nanoplates were modified by different functional groups, including the amino group, carboxyl group, hydroxyl group, and sulfhydryl group (NH-CoO, COOH-CoO, OH-CoO, and SH-CoO). And the modified CoO nanoplates were characterized by XRD, SEM, TEM, XPS, FTIR, TG, and the Zeta potential, verifying the successful modification of different functional groups. Their mimetic peroxidase properties and kinetics process were further studied and showed that the order of their catalytic activities was as follows: NH-CoO > SH-CoO > COOH-CoO > pure CoO > OH-CoO, and the catalysis of modified CoO nanozymes all followed Michaelis-Menten kinetics. The results indicated that the different functional groups changed their electron transfer ability, and further affected their catalytic activity. HO detection was selected as an application model system to evaluate the modified CoO nanozymes. Compared with other CoO nanozymes, a wider linear range from 0.01 to 40 mmol L and a lower detection limit of 1.5 μmol L was constructed with NH-CoO nanozymes. The results suggested that surface modification by functional groups was a robust strategy to improve the application of CoO nanozymes. The enhanced catalytic activity and good biocompatibility of modified CoO nanozymes provided valuable materials for the relative application, such as medical detection and antioxidation.
基于纳米材料的模拟酶，被称为纳米酶，在最近几十年受到了越来越多的关注；然而，它们缺乏生物相容性限制了其在生物医学中的应用，而这可以通过表面修饰来解决。在这项工作中，通过不同的官能团（包括氨基、羧基、羟基和巯基）对 CoO 纳米板进行了修饰，包括 NH-CoO、COOH-CoO、OH-CoO 和 SH-CoO。通过 XRD、SEM、TEM、XPS、FTIR、TG 和 Zeta 电位对修饰后的 CoO 纳米板进行了表征，验证了不同官能团的成功修饰。进一步研究了它们模拟过氧化物酶的性质和动力学过程，结果表明它们的催化活性顺序如下：NH-CoO > SH-CoO > COOH-CoO > 纯 CoO > OH-CoO，修饰后的 CoO 纳米酶的催化均遵循米氏动力学。结果表明，不同的官能团改变了它们的电子传递能力，从而进一步影响了它们的催化活性。选择 HO 检测作为应用模型系统来评估修饰后的 CoO 纳米酶。与其他 CoO 纳米酶相比，NH-CoO 纳米酶构建了一个更宽的线性范围（0.01 至 40 mmol L）和更低的检测限（1.5 μmol L）。结果表明，通过官能团进行表面修饰是提高 CoO 纳米酶应用的一种稳健策略。修饰后的 CoO 纳米酶具有增强的催化活性和良好的生物相容性，为相对应用提供了有价值的材料，例如医学检测和抗氧化。
