Wang Yu, Liu Yumeng, Cao Lidong, Xiao Zunqiang, Lu Yi, Zhang Xin
Department of Colorectal Surgery, Ningbo Medical Center Li Huili Hospital (Li Huili Hospital affiliated to Ningbo University), Ningbo 315000, China.
School of Public Health, Hangzhou Medical College, Hangzhou 311399, China.
ACS Omega. 2025 Feb 10;10(7):6359-6367. doi: 10.1021/acsomega.4c03460. eCollection 2025 Feb 25.
Identification of biological antioxidants is of vital importance because of the essential role of antioxidants in keeping the balance of various diseases. Here, we designed a simple, rapid, and low-cost nanozyme sensing array for colorimetric identification of multiple antioxidants based on Au nanoparticles (Au NPs) synthesized via a facile and green aqueous phase method. In the presence of HO, Au NPs possessed peroxidase-like catalytic activity and could effectively catalyze the color-less 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized product with comparable enzyme kinetics parameters. The function of the colorimetric sensing array was based on the inhibitory effect of diverse antioxidants on the chromogenic system to varying degrees under different pH conditions, resulting in various "turn-off" colorimetric signal responses. Based on the developed sensing array, four kinds of antioxidants with various concentrations and different proportions of the mixtures were successfully discriminated from each other with the aid of principal component analysis. Moreover, the sensing array showed good performance in differentiating antioxidants in human serum samples, which broaden the analytical application of Au NPs. Compared with the existing sensing array, the single Au NP-based sensing array significantly simplified the sensing element and detection process, opening a new avenue for biological molecule identification in real complex samples.
由于抗氧化剂在维持各种疾病平衡中起着至关重要的作用，因此生物抗氧化剂的鉴定具有极其重要的意义。在此，我们设计了一种简单、快速且低成本的纳米酶传感阵列，用于基于通过简便绿色水相法合成的金纳米颗粒（Au NPs）比色识别多种抗氧化剂。在过氧化氢（HO）存在下，Au NPs具有类似过氧化物酶的催化活性，并且能够以可比的酶动力学参数有效地将无色的3,3',5,5'-四甲基联苯胺（TMB）催化为蓝色氧化产物。比色传感阵列的功能基于不同抗氧化剂在不同pH条件下对显色体系有不同程度的抑制作用，从而产生各种“关闭”的比色信号响应。基于所开发的传感阵列，借助主成分分析成功区分了四种不同浓度及不同比例混合物的抗氧化剂。此外，该传感阵列在区分人血清样品中的抗氧化剂方面表现良好，拓宽了Au NPs的分析应用范围。与现有的传感阵列相比，基于单一Au NP的传感阵列显著简化了传感元件和检测过程，为实际复杂样品中的生物分子识别开辟了一条新途径。
