Department of Chemistry, Myongji University, Yongin 03674, South Korea.
Department of Biotechnology, Yonsei University, Seoul 03722, South Korea.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1493-1498. doi: 10.1021/acsabm.0c01377. Epub 2021 Jan 11.
A label-free biosensor is described based on the Raman spectroscopic signatures of monolayer graphene, which are modified in the compartment of cancer cells because of electron-phonon coupling in monolayer graphene. Specifically, the Raman spectra of electrostatically gated monolayer graphene on SiO/Si substrates, in the voltage range from 0 to 5 V, were studied in the absence and the presence of cancer cells. Density functional theory simulations afforded a correlation between cancer cells and the observed Raman spectra, through the regulation of the intensities of the G and 2D Raman vibrational modes with applied voltage. The C-H and N-H bonds of phenylalanine enabled the detection of this biosensing activity. Significantly, this detection can be carried out even in the absence of cancer cell-culturing steps.
本文描述了一种基于单层石墨烯拉曼光谱特征的无标记生物传感器，由于单层石墨烯中的电子-声子耦合，其在癌细胞的隔间中发生了修饰。具体来说，在不存在和存在癌细胞的情况下，研究了 SiO2/Si 衬底上电栅控单层石墨烯在 0 到 5 V 电压范围内的拉曼光谱。通过与施加电压相关联的 G 和 2D 拉曼振动模式强度的调节，密度泛函理论模拟提供了癌细胞与观察到的拉曼光谱之间的相关性。苯丙氨酸的 C-H 和 N-H 键使这种生物传感活性的检测成为可能。值得注意的是，即使在没有癌细胞培养步骤的情况下，也可以进行这种检测。
