Influence of bacteriological media constituents on the reproduction of Salmonella enteritidis bacteriophages.

Three different Salmonella enteritidis phages were isolated and purified from raw sewage by agar-layer technique. The sensitivity of the host organisms toward phages was changed when they were grown on different bacteriological media. The effect of single components of the medium on phage reproduction was determined by the omission of that substance from the medium. CaCl2,MgSO4, and glycerol each had a pronounced stimulatory effect on the phage reproduction, while bile salts had a profound inhibitory effect. The inhibitory effect of bile salts on phage growth was much greater on one strain of Salmonella enteritidis than on the other.