Yildirim Zeliha, Sakіn Tuba, Çoban Fatma
Niğde Ömer Halisdemir University , Faculty of Engineering, Department of Food Engineering, Niğde , Turkey.
Acta Biol Hung. 2018 Sep;69(3):350-369. doi: 10.1556/018.68.2018.3.10.
The objectives of this study were to isolate, purify and determine host range of lytic bacteriophages infecting foodborne the pathogen Salmonella Typhimurium and S. Enteritidis. River/stream water, sewage, raw foods, wastewater from food processing plants, slaughterhouse and fish farms and water from troughs were used for the screening of bacteriophages. The richest sources in terms of phages infecting S. Typhimurium and Enteritidis were found to be sewage, wastewaters of slaughterhouse, food processing and fisheries and streams. A total of 33 S. Typhimurium and 56 S. Enteritidis phages were isolated and purified from the samples. It was demostrated that host ranges of the isolated phages were quite wide. The numbers of bacteria types inhibited by S. Typhimurium or Enteritidis phages were changed among 1-15 and 1-19, respectively. It was found that 75.8% (25 out of 33) and 83.93% (47 out of 56) of isolated S. Typhimurium or Enteritidis phages formed clear plaques and were capable of lysing at least six or two Salmonella serovars. Beside Salmonella serovars, some S. Typhimurium (15 out of 33, 45.5%) and S. Enteritidis phages (5 out of 56, 8.93%) were also infective against E. coli strains. The host ranges of S. Typhimurium phages were wider than those of S. Enteritidis.
本研究的目的是分离、纯化并确定感染食源性病原体鼠伤寒沙门氏菌和肠炎沙门氏菌的裂解性噬菌体的宿主范围。河水/溪水、污水、生食、食品加工厂废水、屠宰场和养鱼场废水以及水槽中的水被用于噬菌体筛选。结果发现，在感染鼠伤寒沙门氏菌和肠炎沙门氏菌的噬菌体方面，最丰富的来源是污水、屠宰场废水、食品加工和渔业废水以及溪流。从这些样本中总共分离并纯化出33株鼠伤寒沙门氏菌噬菌体和56株肠炎沙门氏菌噬菌体。结果表明，分离出的噬菌体的宿主范围相当广泛。鼠伤寒沙门氏菌或肠炎沙门氏菌噬菌体抑制的细菌类型数量分别在1至15种和1至19种之间变化。结果发现，75.8%（33株中的25株）的鼠伤寒沙门氏菌噬菌体和83.93%（56株中的47株）的肠炎沙门氏菌噬菌体形成了清晰的噬菌斑，并且能够裂解至少六种或两种沙门氏菌血清型。除了沙门氏菌血清型外，一些鼠伤寒沙门氏菌噬菌体（33株中的15株，45.5%）和肠炎沙门氏菌噬菌体（56株中的5株，8.93%）也能感染大肠杆菌菌株。鼠伤寒沙门氏菌噬菌体的宿主范围比肠炎沙门氏菌噬菌体的更广。
