Carey-Smith Gwyneth V, Billington Craig, Cornelius Angela J, Hudson J Andrew, Heinemann Jack A
Food Safety Programme, ESR Ltd, Ilam, Christchurch, New Zealand.
FEMS Microbiol Lett. 2006 May;258(2):182-6. doi: 10.1111/j.1574-6968.2006.00217.x.
Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sample of screened sewage. Phage FGCSSa1 had the broadest host range infecting six of eight Salmonella isolates and neither of two Escherichia coli isolates. Under optimal growth conditions for S. Enteritidis PT160, phage infection resulted in a burst size of 139 PFU but was apparently inactive at a temperature typical of stored foods (5 degrees C), even at multiplicity of infection values in excess of 10 000. While neither isolate had characteristics that would make them candidates for biocontrol of Salmonella spp. in foods, phage FGCSSa1 behaved unusually when grown on two Salmonella serotypes at 37 degrees C in that the addition of phages appeared to retard growth of the host, presumably by the lysis of a fraction of the host cell population.
使用含有三种沙门氏菌血清型的软琼脂覆盖层从污水中分离出感染沙门氏菌属的噬菌体，并评估它们控制食源沙门氏菌的潜力。从一份经过筛选的污水样本中获得了两种根据噬菌斑形态、结构和宿主范围定义的不同噬菌体。噬菌体FGCSSa1具有最广泛的宿主范围，可感染八株沙门氏菌中的六株，而两株大肠杆菌分离株均未被感染。在肠炎沙门氏菌PT160的最佳生长条件下，噬菌体感染导致的裂解量为139个噬菌斑形成单位（PFU），但在典型的储存食品温度（5摄氏度）下显然无活性，即使感染复数超过10000。虽然这两种分离株都没有具备使其成为食品中沙门氏菌生物防治候选菌株的特性，但噬菌体FGCSSa1在37摄氏度下于两种沙门氏菌血清型上生长时表现异常，因为添加噬菌体似乎会阻碍宿主生长，推测是由于一部分宿主细胞群体被裂解。
