Department of Biomedical Sciences, College of Veterinary Medicine, Corvallis, OR, USA.
Department of Microbiology, College of Sciences Oregon State University, Corvallis, OR, USA.
J Med Microbiol. 2022 Jan;71(1). doi: 10.1099/jmm.0.001467.
Pulmonary infections caused by organisms of the complex are increasingly prevalent in populations at risk, such as patients with cystic fibrosis, bronchiectasis and emphysema. infection of the lung is not observed in immunocompetent individuals, which raises the possibility that the compromised lung environment is a suitable niche for the pathogen to thrive in due to the overproduction of mucus and high amounts of host cell lysis. Evaluate the ability of to form biofilm and grow utilizing conditions as seen in immunocompromised lungs of patients. We compared biofilm formation and protein composition in the presence and absence of synthetic cystic fibrosis medium (SCFM) and evaluated the bacterial growth when exposed to human DNA. is capable of forming biofilm in SCFM. By eliminating single components found in the medium, it became clear that magnesium works as a signal for the biofilm formation, and chelation of the divalent cations resulted in the suppression of biofilm formation. Investigation of the specific proteins expressed in the presence of SCFM and in the presence of SCFM lacking magnesium revealed many different proteins between the conditions. also exhibited growth in SCFM and in the presence of host cell DNA, although the mechanism of DNA utilization remains unclear. conditions mimicking the airways of patients with cystic fibrosis appear to facilitate establishment of infection, and elimination of magnesium from the environment may affect the ability of the pathogen to establish infection.
复合体引起的肺部感染在高危人群中越来越普遍，如囊性纤维化、支气管扩张和肺气肿患者。免疫功能正常的个体不会感染肺部，这表明由于黏液过度产生和宿主细胞大量裂解，受损的肺部环境可能是病原体滋生的合适小生境。评估 形成生物膜并利用 条件生长的能力，如在免疫功能低下的患者肺部中观察到的那样。我们比较了在存在和不存在合成囊性纤维化培养基 (SCFM) 时生物膜形成和蛋白质组成，并评估了暴露于人 DNA 时细菌的生长情况。能够在 SCFM 中形成生物膜。通过消除培养基中发现的单个成分，很明显镁作为生物膜形成的信号起作用，并且二价阳离子的螯合导致生物膜形成受到抑制。在存在 SCFM 和缺乏镁的 SCFM 存在下表达的特定蛋白质的研究揭示了两种条件之间存在许多不同的蛋白质。在 SCFM 和存在宿主细胞 DNA 的情况下也表现出生长，尽管 DNA 利用的机制尚不清楚。模拟囊性纤维化患者气道的 条件似乎有利于 感染的建立，并且从环境中去除镁可能会影响病原体建立感染的能力。
