Keefe Bailey, Leestemaker-Palmer Amy, Bermudez Luiz E
Department of Biomedical Sciences, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States.
Department of Microbiology, College of Sciences, Oregon State University, Corvallis, OR, United States.
Front Immunol. 2025 Mar 7;16:1508584. doi: 10.3389/fimmu.2025.1508584. eCollection 2025.
is a pathogen recently associated with patients with chronic lung conditions such as bronchiectasis and cystic fibrosis. is an environmental bacterium but recent evidence suggests that the pathogen is also transmitted from host-to-host. Because is known to form biofilms on the respiratory mucosa the release of bacteria from the biofilm becomes an important aspect on the transmission of the infection.
A biofilm releasing system was established. A transposon library of was then screened to identify genes associated with the release from biofilms.
Several enzymes and genes of unidentified function were linked with the ability to detach from the biofilm. It was also shown that detached bacteria were increased capable of establish a new biofilm, attach to epithelial cells, and infect macrophages. To determine the surface molecules linked with the ability to infect new hosts, a surface proteomic was performed, showing that detaching bacteria express many proteins do not present in biofilm bacteria.
Detached , one of the possible infectious phenotypes, contains specific proteins and lipids in the surface that facilitate the infection of new hosts. In addition, we identified many small proteins that have the likelihood to be associated with the release of the biofilm bacteria.
[病原体名称]是一种最近与支气管扩张和囊性纤维化等慢性肺部疾病患者相关的病原体。它是一种环境细菌，但最近的证据表明该病原体也在人与人之间传播。由于已知[病原体名称]会在呼吸道黏膜上形成生物膜，生物膜中细菌的释放成为感染传播的一个重要方面。
建立了一个生物膜释放系统。然后筛选[病原体名称]的转座子文库，以鉴定与从生物膜释放相关的基因。
几种酶和功能不明的基因与从生物膜脱离的能力有关。还表明，脱离的细菌更有能力形成新的生物膜、附着在上皮细胞上并感染巨噬细胞。为了确定与感染新宿主能力相关的表面分子，进行了表面蛋白质组学分析，结果表明脱离的细菌表达许多生物膜细菌中不存在的蛋白质。
脱离的[病原体名称]是可能的感染表型之一，其表面含有特定的蛋白质和脂质，有助于感染新宿主。此外，我们鉴定出许多可能与生物膜细菌释放相关的小蛋白质。
