基于二维图像的菌落生物膜特性分析。
Characterization of colony-biofilms based on bi-dimensional images.
机构信息
Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier , Montpellier, France.
INSERM, IRIM , Montpellier, France.
出版信息
Antimicrob Agents Chemother. 2023 Sep 19;67(9):e0040223. doi: 10.1128/aac.00402-23. Epub 2023 Aug 11.
Abstract
biofilm aggregates have been shown in the lungs of cystic fibrosis patients and are often tolerant to drugs. Herein, we analyzed bi-dimensional images of either fluorescent or Congo red-stained colony-biofilms grown on a membrane to monitor growth and shape of smooth and rough variants. These colony-biofilms responded differently to rifabutin and bedaquiline, thus highlighting the importance of the morphotype to properly address antibiotic treatment in patients with biofilm-related infections.
摘要
生物膜聚集体已在囊性纤维化患者的肺部中显现出来，并且通常对药物具有耐受性。在此，我们分析了在膜上生长的荧光或刚果红染色的菌落生物膜的二维图像，以监测光滑和粗糙变体的生长和形状。这些菌落生物膜对利福布汀和贝达喹啉的反应不同，因此突出了形态对正确解决与生物膜相关感染患者的抗生素治疗的重要性。
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