Yokochi T, Oka K, Iwata H, Miyadai T, Kimura Y, Kato N
Department of Microbiology, Fukui Medical School.
Microbiol Immunol. 1987;31(10):1025-32. doi: 10.1111/j.1348-0421.1987.tb01335.x.
The histological localization and biochemical properties of the autoantigens relevant to experimental autoimmune ophthalmitis and thyroiditis were studied using sera from mice hyperimmunized with the corresponding tissue extract of syngeneic mice and Klebsiella O3 lipopolysaccharide (KO3 LPS) as a potent adjuvant. Specific antigens were detected in the lens of the eyeball by immunofluorescence test with sera from mice in which ophthalmitis had been induced and the antigens were lenticular proteins with molecular weights (MW) of 15,000 (15K) to 25K, and 45K. The lenticular proteins with MW of 15K to 25K correspond to the subunits of crystalline. These findings clearly demonstrated that our experimental model for autoimmune ophthalmitis was classified as the lens-induced uveitis. The colloids of the thyroid follicles and the follicular cells were markedly stained by sera from mice in which thyroiditis had been induced. One of the autoantigens detected in the thyroid gland was biochemically consistent with a thyroglobulin subunit. It was also shown that these autoantigens detected in the present study were organ-specific but not species-specific. The nature of autoantigens in the eye and the thyroid gland is discussed.
使用同基因小鼠相应组织提取物和肺炎克雷伯菌O3脂多糖（KO3 LPS）作为强效佐剂对超免疫小鼠的血清进行研究，以探讨与实验性自身免疫性眼炎和甲状腺炎相关的自身抗原的组织学定位和生化特性。通过对已诱发眼炎的小鼠血清进行免疫荧光试验，在眼球晶状体中检测到特异性抗原，这些抗原是分子量（MW）为15,000（15K）至25K以及45K的晶状体蛋白。分子量为15K至25K的晶状体蛋白对应于晶状体蛋白的亚基。这些发现清楚地表明，我们的自身免疫性眼炎实验模型被归类为晶状体诱导的葡萄膜炎。甲状腺滤泡的胶体和滤泡细胞被已诱发甲状腺炎的小鼠血清显著染色。在甲状腺中检测到的一种自身抗原在生化性质上与甲状腺球蛋白亚基一致。研究还表明，本研究中检测到的这些自身抗原是器官特异性的而非物种特异性的。本文讨论了眼和甲状腺中自身抗原的性质。
