Zhou You, Liao Shenglong, Tao Xinglei, Xu Xiao-Qi, Hong Quan, Wu Di, Wang Yapei
Department of Chemistry, Renmin University of China, Beijing 100872, China.
Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing 100853, China.
ACS Appl Bio Mater. 2018 Aug 20;1(2):502-510. doi: 10.1021/acsabm.8b00230. Epub 2018 Jul 25.
The shortage of tissue resources is currently a serious challenge that limits the clinical therapy to patients with tissue loss or end-stage organ failure. The booming development of 3D printing offers unprecedented hope for tissue engineering since it can construct cells and biomaterials into a 3D tissue-mimicking object with precise control over size and shape. However, it is still challenging to fabricate artificial living tissues or organs due to the extreme complexity of biological tissues. Herein, we propose a new concept of spider-inspired 3D printing technique (SI-3DP) for continuous multicomponent 3D printing based on in situ gelation at a multibarrel printing nozzle. The printing process allows for rapid construction of 3D architectures composed of different inks in the desired position. To present the potential in biomedical applications, the SI-DIP also prints vessel-like hollow hydrogel microfibers and cell-laden hollow fibers, indicating good biocompatibility of this technique. The newly developed SI-3DP technique is envisioned to promote the development of next-generation complex biofabrication.
组织资源的短缺是目前一个严峻的挑战，它限制了对组织缺损或终末期器官衰竭患者的临床治疗。3D打印的蓬勃发展为组织工程带来了前所未有的希望，因为它能够将细胞和生物材料构建成具有精确尺寸和形状控制的3D组织模拟物。然而，由于生物组织极其复杂，制造人工活组织或器官仍然具有挑战性。在此，我们提出了一种受蜘蛛启发的3D打印技术（SI-3DP）的新概念，用于基于多喷头打印喷嘴原位凝胶化的连续多组分3D打印。该打印过程允许在所需位置快速构建由不同墨水组成的3D结构。为了展示其在生物医学应用中的潜力，SI-DIP还打印了血管样中空水凝胶微纤维和载细胞中空纤维，表明该技术具有良好的生物相容性。新开发的SI-3DP技术有望推动下一代复杂生物制造的发展。
