Instituto da Primeira Infância (IPREDE), Fortaleza, Ceará, Brazil; Departamento de Saúde Materno Infantil, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Int J Biol Macromol. 2022 Feb 28;199:243-251. doi: 10.1016/j.ijbiomac.2022.01.011. Epub 2022 Jan 10.
The lack of specific pharmacological therapy for Autistic Spectrum Disorder (ASD) and its clinical heterogeneity demand efforts directed toward the identification of biomarkers to aid in diagnosis. Proteomics offers a new perspective for studying the altered proteins associated with autism spectrum disorders (ASD) and we have saliva as an easy-to-collect biological fluid with important biomolecules for investigating biomarkers in various diseases. In this sense, saliva could be used to identify potential biomarkers of ASD. In the current work, saliva samples were collected from children with different degrees of ASD and healthy children and proteomics approaches were applied to generate data on differentially expressed proteins between groups which will serve as a basis for future validation studies as protein markers. Data are available via ProteomeXchange with identifier PXD030065. As results, 132 proteins were present in 80% of the saliva pools of all analyzed groups. Twenty-five proteins were identified as overexpressed in the group of severe and mild/moderate ASD carriers, among which, eight were identified as potential biomarkers for ASD.
自闭症谱系障碍（ASD）缺乏特定的药物治疗，且其临床表现具有异质性，这促使我们努力寻找生物标志物以辅助诊断。蛋白质组学为研究与自闭症谱系障碍（ASD）相关的异常蛋白提供了新的视角，而唾液作为一种易于采集的生物流体，其中含有多种重要生物分子，可用于研究各种疾病的生物标志物。从这个意义上说，唾液可用于鉴定自闭症的潜在生物标志物。在本研究中，我们收集了不同程度自闭症儿童和健康儿童的唾液样本，并应用蛋白质组学方法生成了组间差异表达蛋白的数据，这些数据将作为未来验证研究的基础，以寻找蛋白标志物。数据可通过 ProteomeXchange 以标识符 PXD030065 进行访问。结果显示，在所有分析组的 80%唾液池中均存在 132 种蛋白。在严重和轻度/中度自闭症携带者组中，有 25 种蛋白被鉴定为过度表达，其中 8 种被鉴定为自闭症的潜在生物标志物。
