Das Lopamudra, Panigrahi Ashok Kumar, Biswal Sashi Bhusan, Bisoi Debasis
Department of Botany, J.K.B.K. Government College, Cuttack, Odisha, India.
Department of Pharmacology, V.S.S. Institute of Medical Sciences and Research, Sambalpur, Odisha, India.
J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1088-S1092. doi: 10.4103/jpbs.jpbs_167_21. Epub 2021 Nov 10.
(NAT) is an ornamental garden plant traditionally used for treating many diseases such as helminthiasis, arthritis, and malaria.
The aim of this study was to validate the ethnobotanical uses of the antimalarial activity of leaves of NAT by tests.
Leaves of NAT were identified and authenticated and phytoconstituents of NAT were identified. The antimalarial activity of NAT was studied in for its schizonticidal activity, repository activity, and curative tests in Swiss albino mice by using (ANKA).
One-way ANOVA was done for comparison of different groups followed by analysis (Tukey-Kramer multiple comparison tests). Level of significance was at < 0.05.
The mean schizonticidal activity of NAT increased from 14.21 to 46.15 ( < 0.01) with doses ranging from 100 to 200 mg/kg compared to 67.29 with that of chloroquine (CQ). The repository activity with NAT doses 100-200 mg/kg increased from 12.91 to 42.85 ( < 0.01) compared to 78.79 in pyrimethamine 1.2 mg/kg/day. In Rane's test, there was chemosuppression in range of 55.50-65.02 ( < 0.01) with NAT in doses of 100-200 mg/kg compared to 74.15 with that of CQ 5 mg/kg.
The antiplasmodial activity of NAT might be like that of artemisinin by producing oxidative stress mostly due to the iridoid glycosides. The active phytoconstituent(s) responsible may be tested individually or in combination both by and studies to identify the active chemical ingredient.
（NAT）是一种园林观赏植物，传统上用于治疗多种疾病，如蠕虫病、关节炎和疟疾。
本研究的目的是通过试验验证NAT叶抗疟活性的民族植物学用途。
对NAT叶进行了鉴定和认证，并鉴定了NAT的植物成分。通过使用伯氏疟原虫（ANKA）在瑞士白化小鼠中研究了NAT的抗疟活性，包括其裂殖体杀灭活性、储存活性和疗效试验。
采用单因素方差分析对不同组进行比较，随后进行事后分析（Tukey-Kramer多重比较检验）。显著性水平设定为P<0.05。
与氯喹（CQ）的67.29相比，NAT的平均裂殖体杀灭活性在剂量为100至200mg/kg时从14.21增加到46.15（P<0.01）。与乙胺嘧啶1.2mg/kg/天的78.79相比，NAT剂量为100 - 200mg/kg时的储存活性从12.91增加到42.85（P<0.01）。在Rane试验中，与CQ 5mg/kg的74.15相比，NAT剂量为100 - 200mg/kg时的化学抑制率在55.50 - 65.02范围内（P<0.01）。
NAT的抗疟原虫活性可能与青蒿素类似，主要通过环烯醚萜苷产生氧化应激。负责的活性植物成分可通过体内和体外研究单独或联合测试，以确定活性化学成分。
