Bharshiv Chandrabhan Kumar, Garg Satish Kumar, Bhatia A K
Department of Pharmacology and Toxicology, U.P. Pandit Deen Dayal Upadhayaya Veterinary and Animal Sciences University, Mathura, Uttar Pradesh, India.
Department of Microbiology, U.P. Pandit Deen Dayal Upadhayaya Veterinary and Animal Sciences University, Mathura, Uttar Pradesh, India.
Indian J Pharmacol. 2016 Jul-Aug;48(4):412-417. doi: 10.4103/0253-7613.186210.
To investigate the immunomodulatory activity of aqueous extract of flowers (NAFE) with particular reference to splenocytes proliferation and induction of cytokines.
Antibody titer was determined by tube agglutination and indirect ELISA assay in four groups of mice-control, antigen alone, and NAFE-treated (400 and 800 mg/kg for 21 days) after immunization with antigen while cellular immunity was studied in three groups of rats (control and NAFE-treated - 400 and 800 mg/kg) following DNCB application. Splenocytes from untreated and NAFE-treated rats were stimulated using concanavalin-A (Con-A) and optical density (OD) and stimulation index were determined. Splenocytes from control rats were also treated with NAFE (50-1600 μg/ml) and Con-A to determine the effect on splenocytes proliferation. Interleukin-2 (IL-2) and IL-6 levels in splenocytes supernatant from control and NAFE-treated rats and following treatment of splenocytes with NAFE (50-1600 μg/ml) were determined using ELISA kits.
Marked to a significant increase in antibody titer by both the methods in NAFE-treated mice and a significant increase in skin thickness in rats after challenge with DNCB, respectively suggested humoral and cell-mediated immunostimulant potential of NAFE. Significant increase in OD and stimulation index following e x vivo and exposure of splenocytes and sensitization with Con-A and significant elevation in IL-2 and IL-6 levels in splenocytes supernantant was also observed after their and exposure to NAFE.
Humoral and cell-mediated immunostimulant activity of NAFE seems to be mediated through splenocytes proliferation and increased production of cytokines, especially IL-2 and IL-6.
研究花朵水提取物（NAFE）的免疫调节活性，特别关注脾细胞增殖和细胞因子诱导情况。
在四组小鼠中通过试管凝集和间接ELISA法测定抗体效价，这四组分别为对照组、仅接种抗原组以及用NAFE处理（400和800mg/kg，共21天）后接种抗原的组；在用二硝基氯苯（DNCB）处理后，在三组大鼠（对照组和用NAFE处理的组 - 400和800mg/kg）中研究细胞免疫。使用刀豆蛋白A（Con-A）刺激未处理和用NAFE处理的大鼠的脾细胞，测定光密度（OD）和刺激指数。还用NAFE（50 - 1600μg/ml）和Con-A处理对照组大鼠的脾细胞，以确定对脾细胞增殖的影响。使用ELISA试剂盒测定对照组和用NAFE处理的大鼠脾细胞上清液中白细胞介素-2（IL-2）和IL-6的水平，以及用NAFE（50 - 1600μg/ml）处理脾细胞后的水平。
NAFE处理的小鼠通过两种方法测定的抗体效价均显著升高，用DNCB攻击后大鼠的皮肤厚度显著增加，分别提示了NAFE的体液免疫和细胞介导免疫刺激潜力。在用Con-A对脾细胞进行体外刺激和致敏后，OD和刺激指数显著增加，在用NAFE处理脾细胞后，脾细胞上清液中的IL-2和IL-6水平也显著升高。
NAFE的体液免疫和细胞介导免疫刺激活性似乎是通过脾细胞增殖以及细胞因子尤其是IL-2和IL-6的产生增加来介导的。
