2型糖尿病患者氧化应激和胰岛素抵抗与葡萄糖-6-磷酸脱氢酶活性的相关性评估

Assessment of Correlation of Oxidative Stress and Insulin Resistance with Glucose-6-Phosphate Dehydrogenase Activity in Type II Diabetes Mellitus Patients.

作者信息

Singh Beena, Kumari Shweta

机构信息

Department of Biochemistry, Career Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

Department of Biochemistry, KGMU, Lucknow, Uttar Pradesh, India.

出版信息

J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1573-S1576. doi: 10.4103/jpbs.jpbs_291_21. Epub 2021 Nov 10.

DOI:10.4103/jpbs.jpbs_291_21

PMID:35018032

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8686861/

Abstract

BACKGROUND

The present study aimed to assess the correlation of oxidative stress glucose-6-phosphate dehydrogenase (G6PD) activity in type II diabetes mellitus (DM) patients.

METHODOLOGY

Forty-eight type II DM patients and healthy subjects were recruited. In all, G6PD activity, protein carbonyl, and total thiol levels were measured.

RESULTS

The mean glycated hemoglobin (HbA1c) was 9.4% in Group I and 5.2% in Group II, G6PD activity was 6.5 U/gHb in Group I and 8.2 U/gHb in Group II, protein carbonyl was 14.2 nmol/mg protein in Group I and 3.5 nmol/mg protein in Group II, and total thiol level was 204.7 μmol/mL in Group I and 318.2 μmol/mL in Group II. In Group I, G6PD activity positively correlated with total thiol ( = 0.62) and negatively correlated with protein carbonyl ( = -0.73) and HbA1C ( = -0.67), protein carbonyl positively correlated with HbA1C ( =0.45) and negatively correlated with total thiol ( = -0.84), and total thiol negatively correlated with HbA1c ( = -0.30).

CONCLUSION

G6PD may be considered a biomarker of oxidative stress and poor glycemic control in diabetic patients.

摘要

背景

本研究旨在评估2型糖尿病（DM）患者氧化应激与葡萄糖-6-磷酸脱氢酶（G6PD）活性之间的相关性。

方法

招募了48名2型糖尿病患者和健康受试者。总共测量了G6PD活性、蛋白质羰基和总巯基水平。

结果

第一组糖化血红蛋白（HbA1c）的平均值为9.4%，第二组为5.2%；第一组G6PD活性为6.5 U/gHb，第二组为8.2 U/gHb；第一组蛋白质羰基为14.2 nmol/mg蛋白质，第二组为3.5 nmol/mg蛋白质；第一组总巯基水平为204.7 μmol/mL，第二组为318.2 μmol/mL。在第一组中，G6PD活性与总巯基呈正相关（= 0.62），与蛋白质羰基呈负相关（= -0.73），与HbA1C呈负相关（= -0.67）；蛋白质羰基与HbA1C呈正相关（=0.45），与总巯基呈负相关（= -0.84）；总巯基与HbA1c呈负相关（= -0.30）。