Singh Beena, Kumari Shweta
Department of Biochemistry, Career Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Department of Biochemistry, KGMU, Lucknow, Uttar Pradesh, India.
J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1573-S1576. doi: 10.4103/jpbs.jpbs_291_21. Epub 2021 Nov 10.
The present study aimed to assess the correlation of oxidative stress glucose-6-phosphate dehydrogenase (G6PD) activity in type II diabetes mellitus (DM) patients.
Forty-eight type II DM patients and healthy subjects were recruited. In all, G6PD activity, protein carbonyl, and total thiol levels were measured.
The mean glycated hemoglobin (HbA1c) was 9.4% in Group I and 5.2% in Group II, G6PD activity was 6.5 U/gHb in Group I and 8.2 U/gHb in Group II, protein carbonyl was 14.2 nmol/mg protein in Group I and 3.5 nmol/mg protein in Group II, and total thiol level was 204.7 μmol/mL in Group I and 318.2 μmol/mL in Group II. In Group I, G6PD activity positively correlated with total thiol ( = 0.62) and negatively correlated with protein carbonyl ( = -0.73) and HbA1C ( = -0.67), protein carbonyl positively correlated with HbA1C ( =0.45) and negatively correlated with total thiol ( = -0.84), and total thiol negatively correlated with HbA1c ( = -0.30).
G6PD may be considered a biomarker of oxidative stress and poor glycemic control in diabetic patients.
本研究旨在评估2型糖尿病（DM）患者氧化应激与葡萄糖-6-磷酸脱氢酶（G6PD）活性之间的相关性。
招募了48名2型糖尿病患者和健康受试者。总共测量了G6PD活性、蛋白质羰基和总巯基水平。
第一组糖化血红蛋白（HbA1c）的平均值为9.4%，第二组为5.2%；第一组G6PD活性为6.5 U/gHb，第二组为8.2 U/gHb；第一组蛋白质羰基为14.2 nmol/mg蛋白质，第二组为3.5 nmol/mg蛋白质；第一组总巯基水平为204.7 μmol/mL，第二组为318.2 μmol/mL。在第一组中，G6PD活性与总巯基呈正相关（= 0.62），与蛋白质羰基呈负相关（= -0.73），与HbA1C呈负相关（= -0.67）；蛋白质羰基与HbA1C呈正相关（=0.45），与总巯基呈负相关（= -0.84）；总巯基与HbA1c呈负相关（= -0.30）。
G6PD可被视为糖尿病患者氧化应激和血糖控制不佳的生物标志物。
