Mahmoud Aida A, Nor El-Din Amal K A
Biochemistry Department, Faculty of Medicine, Sohag University, Sohag, Egypt.
Internal Medicine Department, Faculty of Medicine, Sohag University, Sohag, Egypt.
J Biomark. 2013;2013:430813. doi: 10.1155/2013/430813. Epub 2013 Dec 22.
Objectives. The aim of the present investigation was to study the activity of glucose-6-phosphate dehydrogenase (G6PD) and correlate its activity to protein oxidation markers in type 2 diabetic patients under poor glycemic control. Methods. G6PD activity, protein carbonyl group concentration, and total thiol group content were measured in blood samples of 40 patients with type 2 diabetes mellitus under poor glycemic control and 20 healthy control subjects. Results. G6PD activity and total thiol group content decreased significantly while glycated hemoglobin (HbA1C) and protein carbonyl group concentration increased significantly in diabetic patients than in the controls (P < 0.001). In addition, Obtained results revealed that, in diabetics, G6PD activity negatively correlated to protein carbonyl and HbA1C (r = -0.77 and -0.65, resp.), while positively correlated to total thiol (r = 0.66) and protein carbonyl negatively correlated to total thiol (r = -0.85), while positively correlated to HbA1C (r = 0.43). Also in controls, G6PD activity negatively correlated to protein carbonyl and HbA1C (r = -0.57 and -0.56, resp.), while positively correlated to total thiol (r = 0.5) and protein carbonyl negatively correlated to total thiol (r = -0.48), while positively correlated to HbA1C (r = 0.68). Conclusions. We concluded that G6PD activity decreased in diabetics than in controls and was negatively correlated to oxidative stress markers and HbA1C. G6PD activity can be taken as a biomarker of oxidative stress and poor glycemic control in type 2 diabetic patients.
目的。本研究旨在探讨血糖控制不佳的2型糖尿病患者体内葡萄糖-6-磷酸脱氢酶（G6PD）的活性，并将其活性与蛋白质氧化标志物进行关联分析。方法。对40例血糖控制不佳的2型糖尿病患者及20例健康对照者的血样进行G6PD活性、蛋白质羰基浓度和总巯基含量的检测。结果。与对照组相比，糖尿病患者的G6PD活性和总巯基含量显著降低，而糖化血红蛋白（HbA1C）和蛋白质羰基浓度显著升高（P < 0.001）。此外，研究结果显示，在糖尿病患者中，G6PD活性与蛋白质羰基及HbA1C呈负相关（r分别为-0.77和-0.65），与总巯基呈正相关（r = 0.66），蛋白质羰基与总巯基呈负相关（r = -0.85），与HbA1C呈正相关（r = 0.43）。在对照组中，G6PD活性也与蛋白质羰基及HbA1C呈负相关（r分别为-0.57和-0.56），与总巯基呈正相关（r = 0.5），蛋白质羰基与总巯基呈负相关（r = -0.48），与HbA1C呈正相关（r = 0.68）。结论。我们得出结论，糖尿病患者的G6PD活性低于对照组，且与氧化应激标志物及HbA1C呈负相关。G6PD活性可作为2型糖尿病患者氧化应激及血糖控制不佳的生物标志物。
