Schneider-Schaulies J, Hünig T, Schimpl A, Wecker E
Eur J Immunol. 1986 Mar;16(3):312-6. doi: 10.1002/eji.1830160319.
Murine splenic T lymphocytes display maximal cellular myc gene (c-myc) expression already 3 h after concanavalin A stimulation and subsequent down-regulation before the onset of DNA synthesis. Stimulation by leucoagglutinin in the presence or absence of interleukin 2 leads to only low initial levels of c-myc-specific RNA which, however, increase later on. A similar pattern of c-myc expression is shown by the Lyt-2+ T cell subpopulation stimulated with either concanavalin A or leucoagglutinin in the presence of interleukin 2. Although [3H]thymidine incorporation was identical, the leucoagglutinin-stimulated Lyt-2+ T cells were void of any demonstrable c-myc-specific RNA at 3 h post-stimulation. Thus, the kinetics of c-myc expression in mouse T lymphocytes are not at all uniform, but depend on the mitogen and the subpopulation. In contrast, levels of c-ras-Ha-specific RNA were always low at early times, always increased towards the onset of DNA synthesis and down-regulation was not observed.
小鼠脾T淋巴细胞在伴刀豆球蛋白A刺激后3小时即显示出最大程度的细胞myc基因（c-myc）表达，随后在DNA合成开始前下调。在有或无白细胞介素2存在的情况下，白细胞凝集素刺激仅导致c-myc特异性RNA的初始水平较低，但随后会升高。在白细胞介素2存在的情况下，用伴刀豆球蛋白A或白细胞凝集素刺激的Lyt-2 + T细胞亚群显示出类似的c-myc表达模式。尽管[3H]胸苷掺入相同，但在刺激后3小时，白细胞凝集素刺激的Lyt-2 + T细胞没有任何可检测到的c-myc特异性RNA。因此，小鼠T淋巴细胞中c-myc表达的动力学根本不统一，而是取决于促有丝分裂原和亚群。相比之下，c-ras-Ha特异性RNA的水平在早期总是很低，总是在DNA合成开始时升高，并且未观察到下调。
