Clinical Medical College of Acupuncture Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
School of Chinese Medicine, Shandong College of Traditional Chinese Medicine, Yantai 264199, Shandong, PR China.
Food Funct. 2022 Feb 7;13(3):1168-1179. doi: 10.1039/d1fo03067a.
The effects of polysaccharides (CFPs) on renal interstitial fibrosis by regulating the phosphatidylinositol-3-hydroxykinase/protein kinase-B/nuclear factor-κB (PI3K/AKT/NF-κB) signaling pathway were investigated and in this research. The common unilateral urethral obstruction (UUO) model was used to examine the renoprotective effect and its mechanism . Compared to the UUO group, CFP administration could ameliorate renal function, inhibit inflammation and fibrosis, and reduce the deposition of the extracellular matrix and epithelial-mesenchymal transition. Mechanistic results indicated that CFPs could inhibit the expression of the total protein of PI3K and the conversion of the AKT and NF-κB p65 phosphorylated proteins, thereby inhibiting the transduction of the PI3K/AKT/NF-κB pathway. In addition, CFP treatment could improve inflammation and fibrosis in HK-2 cells induced by TGF-β1, and its mechanism was also verified to inhibit the PI3K/Akt/NF-κB signaling pathway. Overall, these results showed that CFP could alleviate renal interstitial fibrosis related to the PI3K/AKT/NF-κB signaling pathway.
本研究探讨了多糖（CFPs）通过调节磷脂酰肌醇-3-羟激酶/蛋白激酶 B/核因子-κB（PI3K/AKT/NF-κB）信号通路对肾间质纤维化的影响，并采用常见的单侧输尿管梗阻（UUO）模型来检验其肾保护作用及其机制。与 UUO 组相比，CFP 给药可改善肾功能，抑制炎症和纤维化，减少细胞外基质的沉积和上皮-间充质转化。机制研究结果表明，CFPs 可抑制 PI3K 总蛋白和 AKT、NF-κB p65 磷酸化蛋白的转化，从而抑制 PI3K/AKT/NF-κB 通路的转导。此外，CFP 处理可改善 TGF-β1 诱导的 HK-2 细胞中的炎症和纤维化，其机制也被证实可抑制 PI3K/Akt/NF-κB 信号通路。综上所述，这些结果表明，CFP 可减轻与 PI3K/AKT/NF-κB 信号通路相关的肾间质纤维化。
