Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Fisiologia e Morfologia Animal, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.
Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Patologia Veterinária, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.
An Acad Bras Cienc. 2022 Jan 7;94(1):e20201583. doi: 10.1590/0001-3765202120201583. eCollection 2022.
Animals corpses in teaching and research institutions could be sources of infection for students and teachers when applied for dissection and surgical practice. This research aimed to evaluate cats' corpses' conservation using a new anatomic technique and vacuum package for seven days, aiming surgical practicing. A 150 mL/kg of alcohol with 5% glycerin and 120 mL/kg of a 20% sodium chloride, 1% nitrite, and 1% sodium nitrate solution was injected on corpses sealed in vacuum packages and put on 0 to 4°C. Skin and jejunum were collected on day 0 (fresh samples/control), and traction analysis was performed for seven consecutive days. On the last day, the liquid in the plastic bags was microbiologically analyzed. There was no statistical difference between control and conservation moments (D1 and D2) in maximal rupture force of the skin, and jejunum was similar to control in D2, D4, and D6. The microbial population did not exceed 6.0x104CFU/mL in total aerobics and 4.8x104CFU/mL in total anaerobes. Biomechanics was not significantly affected, and the microbiological count was low during conservation, demonstrating the possible effectiveness of this anatomical technique for surgery training.
在教学和研究机构中，动物尸体在用于解剖和手术实践时，可能成为学生和教师感染的来源。本研究旨在评估一种新的解剖技术和真空包装，以在 7 天内保存猫尸体，用于手术实践。将密封在真空袋中的尸体注入 150 毫升/千克的酒精（含 5%甘油）和 120 毫升/千克的 20%氯化钠、1%亚硝酸盐和 1%硝酸钠溶液，并将其置于 0 至 4°C。在第 0 天（新鲜样本/对照）采集皮肤和空肠样本，并连续 7 天进行拉伸分析。在最后一天，对塑料袋中的液体进行微生物分析。皮肤的最大破裂力在对照和保存期（D1 和 D2）之间没有统计学差异，而空肠在 D2、D4 和 D6 时与对照相似。总需氧菌和总厌氧菌的微生物总数均未超过 6.0x104CFU/mL 和 4.8x104CFU/mL。生物力学没有受到显著影响，保存期间的微生物计数较低，表明这种解剖技术在手术培训中可能有效。
