Department of Animal Morphology and Physiology, São Paulo State University (UNESP), Jaboticabal, Brazil.
Anat Histol Embryol. 2021 May;50(3):588-593. doi: 10.1111/ahe.12665. Epub 2021 Feb 23.
The use of biological membranes in surgeries is a reality for years, and one of the most used is the bovine pericardium, so the purpose of this research is to describe the bovine pericardium's biomechanics by comparing two directions of a test, one parallel to the longitudinal heart axis and one perpendicular. 20 adult bovine pericardium were tested for the maximum rupture force and rupture elongation, collecting four samples of each pericardium direction. In phase 2, eight pericardia were conserved for 4 months in a 98% glycerine solution, and the solution in which they were submerged was microbiologically analysed monthly. The Mann-Whitney test was used; there was a very significant difference between the perpendicular and parallel groups (p = .0001). The T test showed no significant difference for the rupture elongation (p = .0938). In pericardium preserved in glycerine, the outliers were removed regarding the maximum rupture force, and a Boxcox transformation was performed (λ = 0.25). Outliers were removed for the rupture elongation, and Bartlett's test (p = .7836), and Cramer-Von Mises (p = .5033) were performed and then, the analysis of variance (p < .0001), followed by the Tukey test at 5%. In the microbiological analysis, there was no presence of microorganisms during conservation. The research has shown that the pericardium collection direction influences its resistance and it can be stored in glycerine for 4 months without losing biomechanical characteristics.
生物膜在手术中的应用已经有多年的历史，其中使用最广泛的是牛心包。因此，本研究的目的是通过比较沿心脏长轴的平行方向和垂直方向两种测试方向来描述牛心包的生物力学特性。对 20 个成年牛心包进行最大破裂力和破裂伸长率测试，每个心包方向采集 4 个样本。在第 2 阶段，将 8 个心包保存在 98%的甘油溶液中 4 个月，并每月对心包所在的溶液进行微生物分析。采用曼-惠特尼检验；垂直组和平行组之间存在非常显著的差异（p=0.0001）。T 检验显示破裂伸长率无显著差异（p=0.0938）。在甘油保存的心包中，去除了最大破裂力的异常值，并进行 Boxcox 变换（λ=0.25）。去除了破裂伸长率的异常值，并进行了巴特利特检验（p=0.7836）和 Cramer-Von Mises 检验（p=0.5033），然后进行方差分析（p<0.0001），接着进行 Tukey 检验（5%）。在微生物分析中，保存过程中没有微生物的存在。研究表明，心包的采集方向会影响其阻力，并且可以在甘油中保存 4 个月而不会失去生物力学特性。
