Biondo-Simões Maria de Lourdes Pessole, Zwierzikowski Jaqueline Alves, Antoria Juliane Castro Duarte, Ioshii Sérgio Ossamu, Robes Rogério Ribeiro
Full Professor. Department of Surgery. Postgraduate Program in Clinical Surgery - Universidade Federal do Paraná - Curitiba (PR), Brazil.
Graduate student. School of Medicine - Universidade Federal do Paraná - Curitiba (PR), Brazil.
Acta Cir Bras. 2022 Jan 5;36(11):e361101. doi: 10.1590/ACB361101. eCollection 2022.
To compare biological compatibility, hemostasis, and adhesion formation between oxidized regenerated cellulose and lyophilized hydrolyzed porcine collagen in liver trauma.
Forty male Wistar rats constituted two groups: group A (oxidized cellulose) and group B (lyophilized hydrolyzed collagen). Standardized liver trauma was made, and the hemostatic agent was applied. Animals in subgroups A7 and B7 were submitted to euthanasia and relaparotomy after seven days, and in subgroups A14 and B14 after 14 days. Macroscopic and microscopic results were evaluated.
There was no fluid in the cavity in any of the animals, and adhesions were present in all of them. In the analysis after seven days, the adhesions were grades 3 or 4 and consisted of omentum, small intestine, and abdominal wall (p<0.05). In both groups, the mesh was surrounded by a capsule, which was not observed after 14 days. In the evaluation after 14 days, adhesions were grades 2 or 3 (p>0.05). The microscopic examination showed subacute and chronic reactions, in both groups and in both timepoints, with similar frequency. The intensity of fibrosis always presented positive scores. Microabscesses and xanthomatous macrophages were observed in both groups.
There was no superiority of one agent over the other.
比较氧化再生纤维素和冻干水解猪胶原蛋白在肝外伤中的生物相容性、止血效果及粘连形成情况。
40只雄性Wistar大鼠分为两组：A组（氧化纤维素）和B组（冻干水解胶原蛋白）。制作标准化肝外伤模型，并应用止血剂。A7和B7亚组的动物在7天后实施安乐死并再次剖腹，A14和B14亚组的动物在14天后实施安乐死并再次剖腹。评估宏观和微观结果。
所有动物的腹腔内均无积液，且均存在粘连。在7天后的分析中，粘连为3级或4级，由大网膜、小肠和腹壁组成（p<0.05）。两组中，网片均被包膜包裹，14天后未观察到包膜。在14天后的评估中，粘连为2级或3级（p>0.05）。显微镜检查显示，在两个组的两个时间点均出现亚急性和慢性反应，频率相似。纤维化强度始终呈现阳性评分。两组均观察到微脓肿和黄色瘤样巨噬细胞。
两种药物均无优于对方的情况。
