Choi Sung-Won, Kang Jieun, Wang Caifeng, Lee Hyun Min, Oh Se-Joon, Pak Kyoungjune, Shin Nari, Lee Il-Woo, Lee Jaebeom, Kong Soo-Keun
Department of Otorhinolaryngology and Pusan National University School of Medicine, Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Republic of Korea.
Department of Cogno-mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea.
ACS Appl Bio Mater. 2020 Feb 17;3(2):1008-1017. doi: 10.1021/acsabm.9b01018. Epub 2020 Jan 28.
Canal wall down (CWD) mastoidectomy is a surgical procedure that is essential to eradicate chronic ear diseases but results into bone defects due to the resulting mastoid cavity. This study investigated the ability of human tonsil-derived mesenchymal stem cells (hTMSCs) combined with a hydroxyapatite (HAp)-chitosan patch to promote osteogenesis in the treatment of postoperative temporal bone defects using an animal model. At 12 weeks postsurgery, the obliteration ratio and bone formation in the tympanic bulla of rats that were grafted with hTMSCs and a hydroxyapatite (HAp)-chitosan patch or not were compared using histopathological observation and computed tomography (CT) imaging. The group of rats that was administered the Patch group and the group of rats that was administered hTMSCs + Patch group both showed significantly remarkable obliteration ratios and bone formation compared with the Sham and hTMSCs groups. Moreover, the hTMSCs + Patch group showed bone formation in both the periphery and the central region of the tympanic bulla cavity, indicating prominently enhanced osteogenesis compared to the Patch group. Altogether, the results showed that combining hTMSCs with a HAp-chitosan patch accelerated osteogenesis for reconstruction of postoperative temporal bone defects. These findings demonstrate that the hTMSCs + Patch scaffolds are promising for treatment of the bone defects in the tissue engineering.
开放式乳突根治术（CWD）是根除慢性耳部疾病的一种必要外科手术，但由于形成乳突腔会导致骨缺损。本研究使用动物模型，调查了人扁桃体来源的间充质干细胞（hTMSCs）与羟基磷灰石（HAp）-壳聚糖贴片联合使用在治疗术后颞骨缺损中促进骨生成的能力。术后12周，通过组织病理学观察和计算机断层扫描（CT）成像，比较了植入hTMSCs和羟基磷灰石（HAp）-壳聚糖贴片或未植入的大鼠鼓泡的闭塞率和骨形成情况。与假手术组和hTMSCs组相比，贴片组大鼠和hTMSCs +贴片组大鼠的闭塞率和骨形成均显著提高。此外，hTMSCs +贴片组在鼓泡腔的周边和中央区域均有骨形成，表明与贴片组相比，骨生成显著增强。总之，结果表明hTMSCs与HAp-壳聚糖贴片联合使用可加速骨生成，用于术后颞骨缺损的重建。这些发现表明，hTMSCs +贴片支架在组织工程中治疗骨缺损方面具有广阔前景。
