Kang Jieun, Oh Se-Joon, Lee Hyun-Min, Jeong Ki-Jae, Shin Nari, Lee Il-Woo, Lee Jaebeom
J Biomed Nanotechnol. 2017 Dec 1;13(12):1715-1724. doi: 10.1166/jbn.2017.2448.
Many artificial materials have been used for mastoid cavity obliteration in canal wall down tympanomastoidectomy. Among the artificial materials, hydroxyapatite is most popular material for mastoid cavity obliteration. Hydroxyapatite is biostable and osteoconductive, but it has poor mechanical properties, especially brittleness. To overcome the limitation, hydroxyapatite has been combined with natural chitosan polymer, which is biocompatible and flexible, to produce a hydroxyapatite-chitosan patch via a sublimation-assisted compression process. We evaluated this hydroxyapatite-chitosan patch for tympanic cavity obliteration in a rat model by comparing it with sham surgery group, bone cement and homologous cartilage. The cavity obliteration ratio and new bone formation in tympanic bullae were determined by computed tomography, histological analysis, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Comparing the hydroxyapatite-chitosan patch with homologous cartilage and bone cement, the tympanic cavity obliteration ratio was superior in the patch group (40.96 ± 7.79%) than other groups (32.23 ± 6.93% and 16.07 ± 6.10%). Even the hydroxyapatite-chitosan patch made no difference in new bone formation ratio ranging from 27.92 ± 11.08 to 29.17 ± 10.96% with other groups. In conclusion, hydroxyapatite-chitosan patch for mastoid cavity obliteration showed easy handling, flexibility, favorable osteogenesis and biostability.
在开放式鼓室乳突切除术中，许多人工材料已被用于乳突腔填充。在这些人工材料中，羟基磷灰石是最常用于乳突腔填充的材料。羟基磷灰石具有生物稳定性和骨传导性，但其力学性能较差，尤其是脆性。为克服这一局限性，羟基磷灰石已与具有生物相容性和柔韧性的天然壳聚糖聚合物相结合，通过升华辅助压缩工艺制备出一种羟基磷灰石-壳聚糖贴片。我们在大鼠模型中通过将这种羟基磷灰石-壳聚糖贴片与假手术组、骨水泥和同种异体软骨进行比较，来评估其用于鼓室填充的效果。通过计算机断层扫描、组织学分析、场发射扫描电子显微镜和能量色散X射线光谱法测定鼓泡内的腔隙填充率和新骨形成情况。将羟基磷灰石-壳聚糖贴片与同种异体软骨和骨水泥进行比较，贴片组的鼓室腔隙填充率（40.96±7.79%）高于其他组（32.23±6.93%和16.07±6.10%）。即使羟基磷灰石-壳聚糖贴片与其他组相比，新骨形成率在27.92±11.08%至29.17±10.96%之间并无差异。总之，用于乳突腔填充的羟基磷灰石-壳聚糖贴片操作简便、柔韧性好、成骨良好且具有生物稳定性。
